Expression and Purification of the Main Component Contained in Camel Milk and Its Antimicrobial Activities Against Bacterial Plant Pathogens

  • Abbas Tanhaeian
  • Farajollah Shahriari Ahmadi
  • Mohammad Hadi Sekhavati
  • Mojtaba Mamarabadi


Lactoferrin is the most dominant protein in milk after casein. This protein plays a crucial role in many biological processes including the regulation of iron metabolism, induction and modulation of the immune system, the primary defense against microorganisms, inhibiting lipid peroxidation and presenting antimicrobial activity against various pathogens such as parasites, fungi, bacteria, and viruses. The major antimicrobial effect of lactoferrin is related to its N-terminal tail where different peptides for instance lactoferricin and lactoferrampin which are important for their antimicrobial abilities are present. The growth rate of bacterial cells in camel milk is lower than that of the cow milk due to having more antimicrobial compounds. In this study, we have fused a codon-optimized partial camel lactoferrcin and lactoferrampin DNA sequences in order to construct a fused peptide via a lysine. This chimeric 42-mer peptide consists of complete and partial amino acid sequence of camel lactoferrampin and lactoferricin, respectively. Human embryonic kidney 293 (HEK-293) cells were used for synthesizing this recombinant peptide. Finally, the antibacterial activities of this constructed peptide were investigated under in vitro condition. The result showed that, all construction, cloning and expression processes were successfully performed in HEK-293. One His-tag tail was added to the chimera in order to optimize the isolation and purification processes and also reduce the cost of production. Additionally, His-tag retained the antimicrobial activity of the chimera. The antimicrobial tests showed that the growth rate in the majority of bacterial plant pathogens, including gram negative and positive bacteria, was inhibited by recombinant chimera as the level of MIC values were evaluated between 0.39 and 25.07 μg/ml for different bacterial isolates.


Lactoferricin Lactoframpin HEK293 Recombinant peptide Plant bacterial pathogens 



We thank Dr. Hesamoddin Shahriari for editing the manuscript.

Funding Information

This PhD project is financially supported by the Deputy of Research and Technology, Ferdowsi University of Mashhad.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Abbas Tanhaeian
    • 1
  • Farajollah Shahriari Ahmadi
    • 1
  • Mohammad Hadi Sekhavati
    • 2
  • Mojtaba Mamarabadi
    • 3
  1. 1.Department of Biotechnology and Plant Breeding, Faculty of AgricultureFerdowsi University of MashhadMashahadIran
  2. 2.Department of Animal Sciences, Faculty of AgricultureFerdowsi University of MashhadMashahadIran
  3. 3.Department of Plant Protection, Faculty of AgricultureFerdowsi University of MashhadMashahadIran

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