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Effects of Microencapsulated Saccharomyces cerevisiae on Growth, Hematological Indices, Blood Chemical, and Immune Parameters and Intestinal Morphology in Striped Catfish, Pangasianodon hypophthalmus

  • Surintorn Boonanuntanasarn
  • Khanittha Ditthab
  • Araya Jangprai
  • Chatsirin Nakharuthai
Article

Abstract

This study investigated the effects of dietary probiotic Saccharomyces cerevisiae in the striped catfish, Pangasianodon hypophthalmus, which is an important aquaculture species. Freeze-dried microencapsulated probiotic S. cerevisiae with guar gum was performed and used for fish feed supplementation. Striped catfish were fed for 120 days with one of three experimental diets: basal diet (control), basal diet supplemented with 106-CFU S. cerevisiae g−1 diet (S. cerevisiae 106), and basal diet supplemented with 108-CFU S. cerevisiae g−1 diet (S. cerevisiae 108). The S. cerevisiae-supplemented diets significantly improved growth performance including growth rate and feed conversion ratio over 120 days of culture period (P < 0.05). The rate of survival was similar in all experimental groups. Supplementation with S. cerevisiae did not significantly affect whole body proximate composition (P > 0.05). In addition, probiotic S. cerevisiae had no effects on hematological indices and blood chemistry values (glucose, cholesterol, triglycerides, protein, albumin, blood urea nitrogen, chloride, calcium, magnesium, iron, and phosphorus) (P > 0.05). However, dietary S. cerevisiae led to increases in humoral immune parameters including total immunoglobulin, lysozyme, and alternative complement activities (P < 0.05). Dietary S. cerevisiae led to increase intestinal villus height in the anterior part of intestine (P < 0.05). Taken together, while the dietary S. cerevisiae had no detectable effects on hematological indices and several metabolic indicators, significant beneficial probiotic effects were observed on rates of growth, feed conversion ratio, and immune parameters.

Keywords

Saccharomyces cerevisiae Striped catfish Pangasianodon hypophthalmus Probiotic Diet Hematology Immune Intestinal morphology 

Notes

Acknowledgements

Dr. Sirilux Chaijamrus is greatly thanked for the valuable advice of microencapsulation methodology. We thank Mr. Sunai Plymee (SUT Farm) for maintaining the fish throughout this work.

Funding Information

This study was supported by grants from the Suranaree University of Technology (SUT), the Office of the Higher Education Commission, the Higher Education Research Promotion and the National Research University Project of Thailand, and the National Research Council of Thailand (SUT3-303-59-24-18).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All animal manipulations were performed in accordance with the ethical principles and guideline for the use of animals (National Research Council of Thailand) and approved by the Suranaree University of Technology Laboratory Animal Use Monitoring Committee.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Animal Production Technology, Institute of Agricultural TechnologySuranaree University of TechnologyNakhon RatchasimaThailand

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