In Vitro Evaluation of Probiotic Potential of Lactic Acid Bacteria Isolated from Yunnan De’ang Pickled Tea
This study aimed to investigate the probiotic potential of lactic acid bacteria (LAB) strains isolated from De’ang pickled tea, a traditional food consumed by the De’ang nationality of Yunnan, China. Twenty-six LAB strains isolated from De’ang pickled tea were subjected to identification based on 16S rRNA gene sequence analysis. Twenty-four belonged to Lactobacillus plantarum, one belonged to Enterococcus casseliflavus, and one belonged to Lactobacillus acidophilus. Eighteen out of 26 LAB strains which showed a higher capability to tolerate simulated gastrointestinal juices were chosen to further evaluate their probiotic properties. Varied adhesive abilities and auto-aggregative capacities of selected LAB strains were dependent on species and even strains. All tested LAB strains were resistant to kanamycin, streptomycin, gentamycin, and vancomycin and sensitive to tetracycline and chloramphenicol. Ten out of the 18 strains are resistant to ampicillin, and the remaining strains are sensitive to ampicillin; 4 out of the 18 strains showed resistance to erythromycin. Compared to reference strain Lactobacillus rhamnosus strain GG, these LAB strains had a greater or comparative antimicrobial activity against Salmonella typhimurium or Escherichia coli. In contrast, eight out of the 18 strains suppressed growth of Shigella flexneri. Two L. plantarum strains, ST and STDA10, not only exhibited good probiotic properties but also showed a good ability of scavenging DPPH and ABTS+. This study suggests that L. plantarum ST and STDA10 could be used as potential probiotics applied in functional foods.
KeywordsPickled tea LAB Gastrointestinal tolerance Antimicrobial activity Antioxidation Probiotics
This study was supported by the National Natural Science Foundation of China (Grant No. 31501496 and 31760448) and Applied Basic Research Projects of Yunnan Province (Grant No. 2017FB064).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Nanba A, Miyagawa K, Omori M, Kato M, Tamura A, Saito H (1998) Non-salted pickled tea (sour tea) in south-east Yunnan in China. Japan Soc Home Econ 49:907–915Google Scholar
- 5.Hiasa M, Kurokawa M, Ohta K, Esumi T, Akita H, Niki K, Yagi Y, Echigo N, Hatakeyama D, Kuzuhara T (2013) Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea. Food Res Int 54(1):72–80. https://doi.org/10.1016/j.foodres.2013.05.036 CrossRefGoogle Scholar
- 7.Tanasupawat S, Pakdeeto A, Thawai C, Yukphan P, Okada S (2007) Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcuss iamensis sp. nov. J Gen Appl Microbiol 53(1):7–15. https://doi.org/10.2323/jgam.53.7 CrossRefGoogle Scholar
- 8.Xiao P, Huang Y, W1 Y, B1 Z, Quan X (2015) Screening lactic acid bacteria with high yielding-acid capacity from pickled tea for their potential uses of inoculating to ferment tea products. J Food Sci Tech 52(10):6727–6734. https://doi.org/10.1007/s13197-015-1803-6
- 9.FAO/WHO (2006) Probiotics in food: health and nutritional properties and guidelines for evaluation. Rome, p 85Google Scholar
- 11.Leatherhead Food International (2006) The international market for functional foods. 3rd ed., Functional food market report (ISBN 1 904007-82-1)Google Scholar
- 18.Ilavenil S, Vijayakumar M, Kim DH, Valan Arasu M, Park HS, Ravikumar S, Choi KC (2015) Assessment of probiotic, antifungal and cholesterol lowering properties of Pediococcus pentosaceus KCC-23 isolated from Italian ryegrass. J Sci Food Agric doi 96(2):593–601. https://doi.org/10.1002/jsfa.7128 CrossRefGoogle Scholar
- 19.Franklin RC, Matthew AW, Jeff A, Michael ND, George ME (2012) Performance standards for antimicrobial susceptibility testing. Twenty-second informational supplement. Clinical and laboratory standards institute (CLSI): Wayne, PA, USAGoogle Scholar
- 20.Tejero-Sariñena S, Barlow J, Costabile A, Gibson GR, Rowland I (2012) In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe 18(5):530–538. https://doi.org/10.1016/j.anaerobe.2012.08.004 CrossRefGoogle Scholar
- 21.Jin D, Chen C, Li L, Lu S, Li Z, Zhou Z, Jing H, Xu Y, Du P, Wang H, Xiong Y, Zheng H, Bai X, Sun H, Wang L, Ye C, Gottschalk M, Xu J (2013) Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis. BMC Microbiol 13(1):141. https://doi.org/10.1186/1471-2180-13-141 CrossRefGoogle Scholar
- 25.Ankolekar C, Johnson D, Pinto Mda S, Johnson K, Labbe R, Shetty K (2011) Inhibitory potential of tea polyphenolics and influence of extraction time against Helicobacter pylori and lack of inhibition of beneficial lactic acid bacteria. J Med Food 14(11):1321–1329. https://doi.org/10.1089/jmf.2010.0237 CrossRefGoogle Scholar
- 26.Zhao D, Shah NP (2016) Lactic acid bacterial fermentation modified phenolic composition in tea extracts and enhanced their antioxidant activity and cellular uptake of phenolic compounds following in vitro digestion. J Funct Foods 20:182–194. https://doi.org/10.1016/j.jff.2015.10.033 CrossRefGoogle Scholar
- 34.Felten A, Barreau C, Bizet C, Lagrange PH, Philippon A (1999) Lactobacillus species identification, H2O2 production, and antibiotic resistance and correlation with human clinical status. J Clin Microbiol 37(3):729–733Google Scholar