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Possible Probiotic Lactic Acid Bacteria Isolated from Oysters (Crassostrea gigas)

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Abstract

We attempted to isolate lactic acid bacteria (LAB) from the marine oyster (Crassostrea gigas) and selected several environmental stress-resistant isolates for the development of a future probiotic adjuvant for marine aquaculture. Twenty-six presumptive LAB isolates were extracted from oysters and screened (by an agar diffusion assay) for antimicrobial activity toward various pathogens: Vibrio parahaemolyticus, Streptococcus iniae, and Edwardsiella tarda. Eight isolates had an antibacterial activity toward V. parahaemolyticus; in particular, 6 isolates showed a growth-inhibitory activity, with inhibition zone diameters > 15 mm. Of these, 5 isolates (JL17, JL18, JL28, HL7, and HL32) were also active against S. iniae and E. tarda. Enterococcus faecium HL7 was selected as the isolate most resistant to environmental stressors: the minimum NaCl, ethanol, and hydrogen peroxide concentrations at which HL7 cells lost their viability were 1.9 M, 11%, and 0.013%, respectively. When an antibiotic sensitivity test was performed on E. faecium HL7, this isolate was found to be resistant to trimethoprim/sulfamethoxazole, cephalothin, ampicillin, rifampin, gentamicin, cefotaxime, cefepime, cefotetan, nalidixic acid, and kanamycin. While the oyster model studies provided indication that E. faecium HL7 could be a good candidate as biocontrol agent against V. vulnificus, further optimization is needed in the actual animal rearing situation.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, South Korea

    Chang-Ho Kang, Takyong Gu & Jae-Seong So

  2. MEDIOGEN, Co., Ltd., Jecheon-si, 27159, South Korea

    Chang-Ho Kang

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Correspondence to Jae-Seong So.

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Kang, CH., Gu, T. & So, JS. Possible Probiotic Lactic Acid Bacteria Isolated from Oysters (Crassostrea gigas). Probiotics & Antimicro. Prot. 10, 728–739 (2018). https://doi.org/10.1007/s12602-017-9315-5

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