The Interactive Effects of Elevated CO2 and Ammonium Enrichment on the Physiological Performances of Saccharina japonica (Laminariales, Phaeophyta)
Environmental challenges such as ocean acidification and eutrophication influence the physiology of kelp species. We investigated their interactive effects on Saccharina japonica (Laminariales, Phaeophyta) under two pH conditions [Low, 7.50; High (control), 8.10] and three NH 4 + concentrations (Low, 4; Medium, 60; High, 120 μM). The degree of variation of pH values in the culture medium and inhibition rate of photosynthetic oxygen evolution by acetazolamide were affected by pH treatments. Relative growth rates, carbon, nitrogen, and the C:N ratio in tissue samples were influenced by higher concentrations of NH 4 + . Rates of photosynthetic oxygen evolution were enhanced under elevated CO2 or NH 4 + conditions, independently, but these two factors did not show an interactive effect. However, rates of NH 4 + uptake were influenced by the interactive effect of increased CO2 under elevated NH 4 + treatment. Although ocean acidification and eutrophication states had an impact on physiological performance, chlorophyll fluorescence was not affected by those conditions. Our results indicated that the physiological reactions by this alga were influenced to some extent by a rise in the levels of CO2 and NH 4 + . Therefore, we expect that the biomass accumulation of S. japonica may well increase under future scenarios of ocean acidification and eutrophication.
KeywordsCO2 eutrophication NH4+ ocean acidification (OA) pH Saccharina japonica
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- Agatsuma Y, Endo H, Yoshida S, Ikemori C, Takeuchi Y, Fujishima H, Nakajima N, Sano M, Kanezaki N, Imai H, Yamamoto N, Kanahama H, Matsubara T, Takahashi S, Isogai T, Taniguchi K (2014) Enhancement of Saccharina kelp production by nutrient supply in the Sea of Japan off southwestern Hokkaido, Japan. J Appl Phycol 26:1845–1852CrossRefGoogle Scholar
- Boderskov T, Schmedes PS, Bruhn A, Rasmussen MB, Nielsen MM, Pedersen MF (2016) The effect of light and nutrient availability on growth, nitrogen, and pigment contents of Saccharina latissima (Phaeophyceae) grown in outdoor tanks, under natural variation of sunlight and temperature, during autumn and early winter in Denmark. J Appl Phycol 28:1153–1165CrossRefGoogle Scholar
- Dawes CJ, Koch EW (1990) Physiological responses of the red algae Gracilaria verrucosa and G. tikvahiae before and after nutrient enrichment. B Mar Sci 46:335–344Google Scholar
- FAO (2017) The state of world fisheries and aquaculture. Food and Agriculture Organization of the United Nations. https://doi.org/www.fao.org/figis/servlet/TabSelector Accessed 7 Sep 2017Google Scholar
- Gattuso JP, Gao K, Lee K, Rost B, Schulz KG (2010) Guide to best practices for ocean acidification research and data reporting. In: Riebesell U, Fabry VJ, Hansson L, Gattuso JP (eds) Approaches and tools to manipulate the carbonate chemistry. Publications Office of the European Union, Luxembourg, pp 41–52Google Scholar
- Gordillo FJ, Niell FX, Figueroa FL (2001) Non-photosynthetic enhancement of growth by high CO2 level in the nitrophilic seaweed Ulva rigida C. Agardh (Chlorophyta). Planta 213:64–70Google Scholar
- Gran G (1952) Determination of the equivalence point in potentiometric titrations of seawater with hydrochloric acid. Oceanol Acta 5:209–218Google Scholar
- Hanelt D, Uhrmacher S, Nultsch W (1995) The effect of photoinhibition on photosynthetic oxygen production in the brown alga Dictyota dichotoma. Plant Biol 108:99–105Google Scholar
- IPCC (2014) Climate Change 2014: synthesis report. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, New York, 151 pGoogle Scholar
- Orr JC, Fabry VJ, Aumont O, Bopp L, Doney SC, Feely RA, Gnanadesikan A, Gruber N, Ishida A, Joos F, Key RM, Lindsay K, Maier-Reimer E, Matear R, Monfray P, Mouchet A, Najjar RG, Plattner GK, Rodgers KB, Sabine CL, Sarmiento JL, Schlitzer R, Slater RD, Totterdell IJ, Weirig MF, Yamanaka Y, Yool A (2005) Anthropogenic ocean acidification over the twenty-first century and its impact on calcifying organisms. Nature 437:681–686CrossRefGoogle Scholar
- Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods for seawater analysis. Pergamon press, NewYork, 173 pGoogle Scholar
- Raven J, Caldeira K, Elderfield H, Hoegh-Guldberg O, Liss P, Riebesell U, Shepherd J, Turley C, Watson A (2005) Ocean acidification due to increasing atmospheric carbon dioxide. The Royal Society, London, 60 pGoogle Scholar