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Selection for resistance, life history traits and the biochemical mechanism of resistance to thiamethoxam in the maize armyworm, Mythimna separata (Lepidoptera: Noctuidae)

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Abstract

The maize armyworm, Mythimna separata is a polyphagous insect pest of sporadic occurrence. Thiamethoxam is a neonicotinoid insecticide used for the management of many pests in many parts of the world. To develop a resistance management strategy, selection for resistance, the larval fitness parameters and the biochemical mechanisms of resistance to thiamethoxam were studied for thiamethoxam-selected and susceptible M. separata strains based on laboratory observations. The results of our bioassay showed that the thiamethoxam-selected strain was 17.03-fold more resistant than the susceptible strain. The thiamethoxam-selected strain had prolonged larval durations, lower pre-pupal weight of males, and a longer development time from egg to adult than the susceptible strain. The biochemical analyses showed that the GST, CarE and cytochrome P450 enzymes are associated with the development of thiamethoxam resistance in the thiamethoxam-selected strain of M. separata. In this study, the occurrence of resistance may cost developmental fitness for the thiamethoxam-selected strain and provide useful information for designing management strategies to delay resistance.

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Acknowledgments

We are grateful to Prof. J. R. Schrock (Emporia State University, USA) for revising the manuscript, and also to Prof. Dr. Hsin Chi for his help. This research was supported by the Special Fund for the Public Interest (Agriculture) (200903052) by The Ministry of Science and Technology and The Ministry of Agriculture of China and the “13115” Sci-Tech Innovation Project of Shaanxi Province (2007ZDKG-14).

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  1. Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hassan Yasoob, Yifan Li & Yalin Zhang

  2. Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

    Naeem Abbas

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  1. Hassan Yasoob
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  2. Naeem Abbas
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Correspondence to Yalin Zhang.

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Yasoob, H., Abbas, N., Li, Y. et al. Selection for resistance, life history traits and the biochemical mechanism of resistance to thiamethoxam in the maize armyworm, Mythimna separata (Lepidoptera: Noctuidae). Phytoparasitica 46, 627–634 (2018). https://doi.org/10.1007/s12600-018-0692-4

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