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Mechanism of luminal ATP activated chloride secretion in a polarized epithelium

  • N. Keating
  • K. Dev
  • A. C. Hynes
  • L. R. Quinlan
Original Paper
  • 38 Downloads

Abstract

There are both secretory and absorptive pathways working in tandem to support ionic movement driving fluid secretion across epithelia. The mechanisms exerting control of fluid secretion in the oviduct is yet to be fully determined. This study explored the role of apical or luminal extracellular ATP (ATPe)-stimulated ion transport in an oviduct epithelium model, using the Ussing chamber short-circuit current (Isc) technique. Basal Isc in oviduct epithelium in response to apical ATPe comprises both chloride secretion and sodium absorption and has distinct temporal phases. A rapid transient peak followed by a sustained small increase above baseline. Both phases of the apical ATPe Isc response are sensitive to anion (HCO3, Cl) and cation (Na+) replacement. Additionally, the role of apical chloride channels, basolateral potassium channels and intracellular calcium in supporting the peak Isc current was confirmed. The role of ATP breakdown to adenosine resulting in the activation of P2 receptors was supported by examining the effects of non-hydrolyzable forms of ATP. A P2YR2 potency profile of ATP = UTP > ADP was generated for the apical membrane, suggesting the involvement of the P2YR2 subtype of purinoceptor. A P2X potency profile of ATP = 2MeSATP > alpha,beta-meATP > BzATP was also generated for the apical membrane. In conclusion, these results provide strong evidence that purinergic activation of apical P2YR2 promotes chloride secretion and is thus an important factor in fluid formation by the oviduct.

Keywords

Oviduct epithelium Chloride secretion ATP Calcium signaling Potassium channels Chloride channels Purinoceptors 

Notes

Funding

This work was partly funded by the Millennium Research Fund, National University of Ireland, Galway.

Compliance with ethical standards

Conflict of interest

All Authors declare no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© The Physiological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • N. Keating
    • 1
  • K. Dev
    • 1
  • A. C. Hynes
    • 1
  • L. R. Quinlan
    • 1
    • 2
  1. 1.Physiology, School of MedicineNational University of Ireland, GalwayGalwayIreland
  2. 2.CÚRAM, Centre for Research in Medical DevicesNUI GalwayGalwayIreland

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