Short-term treadmill exercise in a cold environment does not induce adrenal Hsp72 and Hsp25 expression
- 208 Downloads
Heat shock proteins (Hsps) have a critical role in maintaining cellular homeostasis and in protecting cells from a range of acute and chronic stressful conditions. Treadmill running exercise results in increased Hsp72 and Hsp25 levels in various tissues and heat production during exercise has been shown to be the main factor for the increased levels of Hsp72 in myocardium. Since the adrenal gland plays a vital role in general response to stress, regulation of Hsps in adrenal glands following stressful events seems to be critical for controlling the whole-body stress response appropriately. This study tested the hypothesis of whether elevation of temperature is solely responsible for exercise-induced adrenal Hsp72 and Hsp25 expression. Female Sprague–Dawley rats (3 months old) were randomly assigned to either a sedentary control group or one of two treadmill-running groups: a cold exercise group run in a cold room at 4 °C (CE), and a warm exercise group run at 25 °C temperature (WE). Animals were run 60 min a day at 30 m min−1 speed for 4 consecutive days following adaptation to treadmill exercise. Exercise resulted in a significant elevation of body temperature only in the WE group (p < 0.05). Adrenal Hsp72 and Hsp25 levels were significantly higher in the WE group compare to the other groups (p < 0.05). These data demonstrated that exercise-related elevations of body temperature could be the only factor for the inductions of adrenal Hsp72 and Hsp25 expression.
KeywordsHsp72 Hsp25 Cold exercise Adrenal Treadmill running
This study was supported by Juntendo University Institute of Health and Sports Science & Medicine. This study was also supported by a Grant-in-Aid for Scientific Research (16300212 and 12480011 to H. Naito) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Compliance with ethical standards
Conflict of interest
The authors report no declarations of interest.
The authors would like to thank Dr. Tahir Hazır for his statistical assistance.
- 28.Ogura Y, Naito H, Akin S, Ichinoseki-Sekine N, Kurosaka M, Kakigi R, Sugiura T, Powers S, Katamoto S, Demirel H (2008) Elevation of body temperature is essential factor for exercise-increased extracellular heat shock protein 72 level in rat plasma. Am J Physiol Regul Integr Comp Physiol 294:1600–1607CrossRefGoogle Scholar
- 34.Skidmore R, Gutierrez JA, Guerriero V Jr, Kregel KC (1995) HSP70 induction during exercise and heat stress in rats: role of internal temperature. Am J Physiol Regul Integr Comp Physiol 268:R92–R97Google Scholar
- 36.Taylor RP, Haris MB, Starnes JW (1999) Acute exercise can improve cardioprotection without increasing heat shock protein content. Am J Physiol Heart Circ Physiol 276:1098–1102Google Scholar