The role of apelin in the healing of water-immersion and restraint stress-induced gastric damage
The objective of this study was to explore the role of apelin in the healing of gastric lesions induced by stress. Male Wistar rats were exposed to water immersion and restraint stress (WIRS) for 6 h with or without the apelin receptor antagonist F13A. The rats were killed on the 1st, 3rd, 5th or 10th day after the end of stress induction. Apelin and hypoxia-inducible factor-1α expression was increased on the 1st day after the end of stress exposure and was decreased daily thereafter. However, F13A retarded the healing of gastric lesions by preventing the improvement of mucosal blood flow, prostaglandin E2 production and vascular endothelial growth factor expression in rats exposed to WIRS. Additionally, F13A increased the gastric 4-hydroxynonenol + malondialdehyde content on the 1st and 3rd days after the end of stress induction but did not affect the change in gastric mucosal nitric oxide levels. In conclusion, apelin may be a regulatory protein involved in the healing mechanism of stress-induced gastric damage.
KeywordsApelin F13A Gastric mucosa Lesion Water immersion and restraint stress
G protein-linked orphan receptor
Water immersion and restraint stress
Apelin receptor antagonist
Gastric mucosal blood flow
Vascular endothelial growth factor
Epidermal growth factor
This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) funded by the Turkish Government (Project number: 110S447).
Compliance with ethical standards
Conflict of interest
The authors report no conflicts of interest.
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