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Processing and biological evaluation of porous HA/poly(methyl methacrylate) hybrid composite

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Abstract

The present study reports the preparation and characterization of dense and porous hydroxyapatite scaffolds for bone regeneration. Porous hydroxyapatite scaffolds were prepared via polymer blend method using poly(methyl methacrylate) as porogenous template to obtain micro- and meso level porosity. The pore size in the sintered ceramics was in the range of 1–50 μm. Hydroxyapatite porous scaffolds were characterized, using X-ray diffraction, Fourier Transformed Infrared Spectroscopy and Scanning Electron Microscopy. The cell adhesion test with human osteoblast cells (SaOS2) confirmed good cytocompatibility of porous composite. Fluorescent staining of osteoblast cells revealed a well-developed cytoskeleton with strong stress fibers. The processing approach in the present investigation to produce porous hydroxyapatite scaffolds with micro and mesopores, can stimulate significant cell adhesion and osteoblast differentiation.

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Acknowledgment

The authors are thankful to Dr. Rajendra K. Bordia, University of Washington, Seattle, USA for helpful discussions at early stage of the material synthesis. This project was partially sponsored by Department of Science and Technology, New Delhi, India, under Fast Track Young Scientist Scheme. The cell culture facility was procured with the funding from Department of biotechnology, Government of India.

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  1. Laboratory for Biomaterials, Department of Material Science and Engineering, Indian Institute of Technology, Kanpur, 208016, India

    Garima Tripathi & Bikramjit Basu

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  1. Garima Tripathi
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  2. Bikramjit Basu
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Correspondence to Bikramjit Basu.

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Tripathi, G., Basu, B. Processing and biological evaluation of porous HA/poly(methyl methacrylate) hybrid composite. Int J Adv Eng Sci Appl Math 2, 161–167 (2010). https://doi.org/10.1007/s12572-011-0032-0

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