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Quality improvement of thermally induced surimi gels by extensive washing for dressed white croaker to remove contamination by body surface mucus proteases

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  • Food Science and Technology
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Abstract

Body surface mucus was scraped off from white croaker Pennahia argentata after washing with two different procedures, suspended in cold water and filtered through a filter paper. The filtrate thus obtained was lyophilized and defined as the fish body surface substances. The relative level of the substances obtained after high-pressure water washing was 28.8% and significantly lower than those obtained after conventional washing (68.8%) and without washing (100%). The breaking strength of thermally induced gels prepared by pre-heating at 65 °C mimicking modori for 60 min after the high-pressure water washing was 3.8 N and was significantly higher than that after the conventional washing (2.6 N) and without washing (2.1 N). These results suggest that rheological properties of thermally induced gels can be improved with an extensive washing of fish material. The degradation of myosin heavy chain was recognized, when the water soluble fraction of the lyophilized powder of the fish body surface mucus was added into the ordinary muscle homogenate at around 60 °C and pH 8.15, suggesting that proteases were present in the fish body surface mucus. Such novel findings are useful for avoiding the modori phenomenon and thus improving the quality of surimi-based products.

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References

  • Akahane T, Chihara S, Yoshida Y, Tsuchiya T, Noguchi S, Ookami H, Matsumoto JJ (1984) Roles of constituent proteins in gel properties of cooked meat gels. Nippon Suisan Gakkaishi 50:1029–1033

    Article  Google Scholar 

  • Aranishi F (1999a) Possible role for cathepsin B and L in bacteriolysis by Japanese eel skin. Fish Shellfish Immunol 8:61–64

    Article  Google Scholar 

  • Aranishi F (1999b) Lysis of pathogenic bacteria by epidermal cathepsins L and B in the Japanese eel. Fish Physiol Biochem 20:37–41

    Article  CAS  Google Scholar 

  • Aranishi F (2000) High sensitivity of skin cathepsins L and B of European eel (Anguilla anguilla) to thermal stress. Aquaculture 182:209–213

    Article  CAS  Google Scholar 

  • Aranishi F, Nakane M (1997) Epidermal proteases of the Japanese eel. Fish Physiol Biochem 16:471–478

    Article  CAS  Google Scholar 

  • Aranishi F, Mano N, Nakane M, Hirose H (1998) Epidermal response of the Japanese eel to environmental stress. Fish Physiol Biochem 19:197–203

    Article  CAS  Google Scholar 

  • Busconi L, Folco EJ, Martone C, Trucco RE, Sanchez JJ (1984) Identification of two alkaline proteases and a trypsin inhibitor from muscle of white croaker (Micropogon opercularis). FEBS Lett 176:211–214

    Article  CAS  Google Scholar 

  • Folco EJ, Busconi L, Martone CB, Sanchez JJ (1988) Multicatalytic proteinase in fish muscle. Arch Biochem Biophys 267:599–605

    Article  CAS  PubMed  Google Scholar 

  • Fukushima H, Yoon SH, Watabe S (2003) Differences in polymer formation through disulfide bonding of recombinant light meromyosin between white croaker and walleye pollock and their possible relation to species-specific differences in thermal unfolding. J Agric Food Chem 51:4089–4095

    Article  CAS  PubMed  Google Scholar 

  • Fukushima H, Satoh Y, Yoon SH, Togashi M, Nakaya M, Watabe S (2005) Rheological properties of fast skeletal myosin rod and light meromyosin from walleye pollack and white croaker: contribution of myosin fragments to thermal gel formation. J Agric Food Chem 53:9193–9198

    Article  CAS  PubMed  Google Scholar 

  • Fukushima H, Okazaki E, Fukuda Y, Watabe S (2007) Rheological properties of selected fish paste at selected temperature pertaining to shaping of surimi-based products. J Food Eng 81:492–499

    Article  CAS  Google Scholar 

  • Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766

    CAS  PubMed  Google Scholar 

  • Hara K, Suzumatsu A, Ishihara T (1988) Purification and characterization of cathepsin B from carp ordinary muscle. Nippon Suisan Gakkaishi 54:1243–1252

    Article  CAS  Google Scholar 

  • Iwata K, Kobashi K, Hase J (1974) Studies on muscle alkaline protease-II. Some enzymatic properties of carp muscular alkaline protease. Nippon Suisan Gakkaishi 40:189–200

    Article  CAS  Google Scholar 

  • Iwata K, Kanna K, Okada M (1977) Kamaboko formation of mackerel and red sea bream myosins. Nippon Suisan Gakkaishi 43:237

    Article  Google Scholar 

  • Kakinuma M, Nakaya M, Hatanaka A, Hirayama Y, Watabe S, Maeda K, Ooi T, Suzuki S (1998) Thermal unfolding of three acclimation temperature-associated isoforms of carp light meromyosin expressed by recombinant DNAs. Biochemistry 37:6606–6613

    Article  CAS  PubMed  Google Scholar 

  • Kanno G, Yamaguchi T, Kishimura H, Yamaha E, Saeki H (2010) Purification and characteristics of trypsin from masu salmon (Oncorhynchus masou) cultured in fresh-water. Fish Physiol Biochem 36:637–645

    Article  CAS  PubMed  Google Scholar 

  • Kimura I, Sugimoto M, Toyoda K, Seki N, Arai K, Fujita T (1991) A study on the cross-linking reaction of myosin in kamaboko suwari gels. Nippon Suisan Gakkaishi 57:1389–1396

    Article  CAS  Google Scholar 

  • Kinoshita M, Toyohara H, Shimizu Y (1990) Purification and properties of a novel latent proteinase showing myosin heavy chain-degrading activity from threadfin-bream muscle. J Biochem 107:587–591

    Article  CAS  PubMed  Google Scholar 

  • Klomklao S, Benjakul S, Visessanguan W, Kishimura H, Simpson BK (2007a) 29 kDa trypsin from the pyloric ceca of Atlantic bonito (Sarda sarda): recovery and characterization. J Agric Food Chem 55:4548–4553

    Article  CAS  PubMed  Google Scholar 

  • Klomklao S, Benjakul S, Visessanguan W, Kishimura H, Simpson BK (2007b) Trypsin from the pyloric caeca of bluefish (Pomatomus saltatrix). Comp Biochem Physiol B: Biochem Mol Biol 148:382–389

    Article  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Makinodan Y, Ikeda S (1969) Studies on fish muscle protease-II. Purification and properties of a proteinase active in slightly alkaline pH range. Nippon Suisan Gakkaishi 35:749–757

    Article  CAS  Google Scholar 

  • Matsuoka Y, Wan J, Ushio H, Watabe S (2013) Thermal gelation properties of white croaker, walleye pollack and deepsea bonefish surimi after suwari treatment at various temperatures. Fish Sci 79:715–724

    Article  CAS  Google Scholar 

  • Nakaya M, Watabe S, Ooi T (1995) Differences in the thermal stability of acclimation temperature-associated carp myosin and its rod isoforms on differential scanning calorimetry. Biochemistry 34:3114–3120

    Article  CAS  PubMed  Google Scholar 

  • Nakaya M, Kakinuma M, Watabe S, Ooi T (1997) Differential scanning calorimetry and CD spectrometry of acclimation temperature-associated types of carp light meromyosin. Biochemistry 36:9179–9184

    Article  Google Scholar 

  • Ni S, Nozawa H, Seki N (1999) The combined effect of transglutaminase and protease inhibitors on the thermal gelation of actomyosin sol from carp and salmon muscles. Fish Sci 65:606–612

    Article  CAS  Google Scholar 

  • Ni S, Nozawa H, Seki N (2001) Effect of pH on the gelation of walleye pollock surimi and carp actomyosin pastes. Fish Sci 67:920–927

    Article  CAS  Google Scholar 

  • Nishimoto S, Hashimoto A, Seki N, Kimura I, Toyoda K, Fujita T, Arai K (1987) Influencing factors on changes in myosin heavy chain and jelly strength of salted meat paste from Alaska pollack during setting. Nippon Suisan Gakkaishi 53:2011–2020 (in Japanese with English abstract)

    Article  CAS  Google Scholar 

  • Nishioka F, Shimizu Y (1983) Recovery of proteins from washings of minced fish meat by pH-shifting method. Nippon Suisan Gakkaishi 49:795–800 (in Japanese with English abstract)

    Article  CAS  Google Scholar 

  • Niwa E, Nakayama T, Hamada I (1983) Effect of setting on the network structure of protein in fish flesh gel. Nippon Suisan Gakkaishi 49:245–249 (in Japanese with English abstract)

    Article  Google Scholar 

  • Numakura T, Seki N, Kimura I, Toyoda K, Fujita T, Takama K, Arai K (1985) Cross-linking reaction of myosin in the fish paste during setting (suwari). Nippon Suisan Gakkaishi 51:1559–1565 (in Japanese with English abstract)

    Article  CAS  Google Scholar 

  • Numakura T, Seki N, Kimura I, Toyoda K, Fujita T, Takama K, Arai K (1987) Effect of quality of surimi on cross-linking reaction of myosin heavy chain during setting. Nippon Suisan Gakkaishi 53:633–639 (in Japanese with English abstract)

    Article  CAS  Google Scholar 

  • Numakura T, Mizoguchi R, Kimura I, Toyoda K, Fujita T, Seki N, Arai K (1989) Changes in gel forming ability and cross-linking ability of myosin heavy chain of Alaska pollack surimi denatured by heat treatment. Nippon Suisan Gakkaishi 55:1083–1090 (in Japanese with English abstract)

    Article  CAS  Google Scholar 

  • Sano T, Noguchi FS, Matsumoto JJ, Tsuchiya T (1989) Role of F-actin in thermal gelation of fish actomyosin. J Food Sci 54:800–804

    Article  CAS  Google Scholar 

  • Satoh Y, Nakaya M, Ochiai Y, Watabe S (2006) Characterization of fast skeletal myosin from white croaker in comparison with that from walleye pollack. Fish Sci 72:646–655

    Article  CAS  Google Scholar 

  • Schneider C, Rasband W, Eliceiri K (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shimizu Y, Machida R, Takenami S (1981) Species variations in the gel-forming characteristics of fish meat paste. Nippon Suisan Gakkaishi 47:95–104

    Article  Google Scholar 

  • Takahashi Y, Kajiwaki T, Itami T, Okamoto T (1987) Enzymatic properties of the bacteriolytic substances in the skin mucus of yellowtail. Nippon Suisan Gakkaishi 53:425–431

    Article  Google Scholar 

  • Ueki N, Wan J, Watebe S (2014) The pepsin digestibility of thermal gel products made from white croaker (Pennahia argentata) muscle in associating with myosin polymerization levels. J Food Sci 79:C2427–C2433

    Article  CAS  PubMed  Google Scholar 

  • Ueki N, Wan J, Watabe S (2016a) Deterioration of white croaker (Pennahia argentata) meat thermally-induced gel products caused by proteolytic enzymes in the contaminated intestine and kidney. Food Chem 199:416–422

    Article  CAS  PubMed  Google Scholar 

  • Ueki N, Wan J, Watabe S (2016b) Proteolytic profiles of walleye pollack (Theragra calcogramma) and white croaker (Pennahia argentata) meats in the presence of intestinal extracts from their own or different fish species. Food Sci Technol Res 22:787–792

    Article  CAS  Google Scholar 

  • Ueki N, Matsuoka Y, Wan J, Watabe S (2018) The effects of endogenous proteases within abdominal muscle parts on the rheological properties of thermally induced gels from white croaker (Pennahia argentata). Food Chem 268:498–503

    Article  CAS  PubMed  Google Scholar 

  • Yanagihara S, Nakaoka H, Hara K, Ishihara T (1991) Purification and characterization of serine proteinase from white croaker skeletal muscle. Nippon Suisan Gakkaishi 57:133–142

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Fish Protein Laboratory, Suzuhiro Kamaboko Honten Co., Ltd., Odawara, Kanagawa, 250-0862, Japan

    Nobuhiko Ueki, Yoko Matsuoka, Jianrong Wan & Shugo Watabe

  2. School of Marine Biosciences, Kitasato University, Minami, Sagamihara, Kanagawa, 252-0373, Japan

    Shugo Watabe

Authors
  1. Nobuhiko Ueki
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  2. Yoko Matsuoka
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  3. Jianrong Wan
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  4. Shugo Watabe
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Correspondence to Nobuhiko Ueki.

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Ueki, N., Matsuoka, Y., Wan, J. et al. Quality improvement of thermally induced surimi gels by extensive washing for dressed white croaker to remove contamination by body surface mucus proteases. Fish Sci 85, 883–893 (2019). https://doi.org/10.1007/s12562-019-01335-x

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  • DOI: https://doi.org/10.1007/s12562-019-01335-x

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