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Antagonistic activity of marine Streptomyces sp. S073 on pathogenic Vibrio parahaemolyticus

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  • Aquaculture
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Abstract

Marine Streptomyces sp. isolate S073 was shown to have strong antagonistic activity towards the pathogenic Vibrio parahaemolyticus using the agar diffusion method. The antagonistic substance(s) secreted into the supernatant was thermostable and non-proteinaceous in nature. S073 was found to produce carboxylate-type siderophores during most of its life cycle using a chrome azurol S assay. The antagonistic activity of S073 was mostly attributed to its higher ability to compete for iron compared with that of V. parahaemolyticus, as deduced from siderophore quantification. Iron supplementation studies indicated that additional mechanisms besides iron competition were simultaneously involved in governing the observed inhibition. Co-culture analysis indicated that S073, although disadvantaged in growth rate, was still competitive in inhibiting vibrios. The promising potential of S073 development as a biocontrol agent in aquaculture was discussed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31300046), the Natural Science Foundation of Guangdong Province (Nos. S2013010013705, 2015A030313319, 2018A030313578), Guangdong Marine and Fishery Bureau Science and Technology Project (No. A201601B05), and the Science and Technology Program of Guangzhou China (No. 201604020029)

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  1. Mingxia Yang and Jun Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, People’s Republic of China

    Mingxia Yang, Jun Zhang, Qiting Liang, Guanxin Pan, Jiachang Zhao, Miao Cui, Qizhong Zhang & Delin Xu

  2. State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Xinqing Zhao

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  1. Mingxia Yang
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Yang, M., Zhang, J., Liang, Q. et al. Antagonistic activity of marine Streptomyces sp. S073 on pathogenic Vibrio parahaemolyticus. Fish Sci 85, 533–543 (2019). https://doi.org/10.1007/s12562-019-01309-z

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