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Molecular characterization and expression profiles of two insulin-like growth factor 1 receptors during fasting and re-feeding in Siniperca chuatsi

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Abstract

Two distinct insulin-like growth factor 1 receptors of the mandarin fish Siniperca chuatsi (scIGF1R1 and scIGF1R2) were cloned and characterized in this study. The complementary DNA of scIGF1R1 [4254 base pairs (bp)] and scIGF1R2 (4248 bp) encoded 1418 and 1416 amino acids, respectively. Both receptors contained a single short transmembrane domain, two receptor L domains and a furin-like cysteine-rich domain. Phylogenetic analysis revealed that scIGF1R1 and scIGF1R2 were clustered in two different branches. Both receptors were expressed in all tissues tested, and the expression levels were relatively high in the gonad, liver and brain. During the first week of starvation, the expression levels of both receptors were increased in the muscle and liver, whereas hepatic scIGF1 expression was decreased. After re-feeding, the expression levels of both receptors were gradually restored to the pre-starvation levels in the liver and muscle. The concentration of serum scIGF1 did not exhibit regular changes compared with the control group during fasting and re-feeding. The varying expression profiles of the scIGF1Rs among tissues indicated that these receptors have distinct, tissue-specific roles. Furthermore, the changes in expression levels of scIGF1, scIGF1R1s during fasting and re-feeding provided evidence to support the link between nutritional status and gene expression related to growth and development.

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Acknowledgments

This project was supported by the Educational Commission of Guangdong Province, China (no. cxzd1104), the Foshan Innovative and Entepreneurial Research Team Program (no. 2014IT100122), the Special Fund for Agro-scientific Research in the Public Interest (no. 201303048), the Science and Technology Planning Project of Guangdong Province (nos. 2007A020300001-1, 2012A020800001, 2008A020100003), the Cooperative Project of Guangdong Province (no. 2011B090400179) and the Agriculture Science Technology Achievement Transformation project (no. 2012GB2E000338). The authors appreciate the support of the Modern Agriculture Talents Support Program (2016-2020), and are grateful to Foshan Nanhai Bairong Aquatic Breeding for providing the breeding ground for early fish seedlings. The authors would also like to thank Linqiang Han, Guiyang Li and Xiuwen Ren for providing assistance during the breeding process.

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  1. Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People’s Republic of China

    Xiaoli Chen, Gongpei Wang, Xue Lu, Peng Xu, Shuang Zeng, Zhi Chen, Qiaoying Zhu & Guifeng Li

  2. Guangdong Haid Group Company Limited, Guangzhou, 511400, People’s Republic of China

    Xue Lu

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  1. Xiaoli Chen
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Correspondence to Guifeng Li.

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Fig. S1

Multiple sequence alignment of the IGF1Rs from Siniperca chuatsi and other teleost species. The amino acid sequences are listed as standard single-letter designations, and gaps are marked by dots. Different background colours represent different conservation, as follows: white non-conserved, blue more than 50% conserved, red more than 80% conserved, and black completely conserved. Sequences used in the alignment: Paralichthys olivaceus fIGF1R1 BAB83669.1, Siniperca chuatsi IGF1R1 MF598471, Sparus aurata IGF1Ra KJ591052.1, Takifugu rubripes IGF1R XM_003967057.2, Neolamprologus brichardi IGF1R1 XP_006789448.1, Oreochromis niloticus IGF1R XP_010731390.1, Cyprinus carpio IGF1Ra AY144591.1, S. chuatsi IGF1R2 MF598472, Larimichthys crocea IGF1R XP_010731390.1, Paralichthys olivaceus fIGF1R2 BAB83670.1, Danio rerio IGF1Rb AAI63581.1 (pptx 1283 kb)

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Chen, X., Wang, G., Lu, X. et al. Molecular characterization and expression profiles of two insulin-like growth factor 1 receptors during fasting and re-feeding in Siniperca chuatsi. Fish Sci 85, 349–360 (2019). https://doi.org/10.1007/s12562-019-01290-7

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