Genome-wide identification and expression of eight fatty acid desaturase genes, and the fatty acid profile, in the marine rotifer Brachionus koreanus fed the alga Tetraselmis suecica

Abstract

Fatty acid desaturases catalyze the formation of a C=C double bond from a C–C single bond in fatty acids, leading to diversification of the fatty acid pool. In this study, we identified the fatty acid desaturase genes in the monogonont marine rotifer Brachionus koreanus, measured the messenger RNA (mRNA) expression, and quantified fatty acid composition under feeding with the alga Tetraselmis suecica. Eight fatty acid desaturase genes were identified and classified by phylogenetic analysis. Fatty acid desaturases in the rotifer B. koreanus were categorized into two Δ4 desaturases, five Δ5/6 desaturases, and one Δ9 desaturase. mRNA expression of B. koreanus under the alga T. suecica-fed condition clearly indicated that transcriptional levels of desaturase genes were increased compared to the non-T. suecica-fed group. Also, the distribution of fatty acids of B. koreanus fed T. suecica was more balanced compared to that of T. suecica. These results provide a better understanding of the role of fatty acid desaturases in B. koreanus fed the alga T. suecica.

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Acknowledgements

We thank two anonymous reviewers for their valuable comments that improved the manuscript. This work was supported by a grant from the National Research Foundation (2018R1D1A1B07050654) to Heum Gi Park.

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Correspondence to Jae-Seong Lee.

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Lee, M., Hagiwara, A., Park, H.G. et al. Genome-wide identification and expression of eight fatty acid desaturase genes, and the fatty acid profile, in the marine rotifer Brachionus koreanus fed the alga Tetraselmis suecica. Fish Sci 85, 397–406 (2019). https://doi.org/10.1007/s12562-018-01286-9

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Keywords

  • Fatty acid desaturase
  • Tandem duplication
  • Unsaturated fatty acid
  • Phylogenetic analysis