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Free radical scavenging ability and structure of a 90-kDa protein from the scallop shell

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  • Chemistry and Biochemistry
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Abstract

We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell.

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Authors and Affiliations

  1. College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, 050-8585, Japan

    Takuya Mitsuhashi, Kohji Ono, Manabu Fukuda & Yasushi Hasegawa

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  1. Takuya Mitsuhashi
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  2. Kohji Ono
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  3. Manabu Fukuda
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  4. Yasushi Hasegawa
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Correspondence to Yasushi Hasegawa.

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Mitsuhashi, T., Ono, K., Fukuda, M. et al. Free radical scavenging ability and structure of a 90-kDa protein from the scallop shell. Fish Sci 79, 495–502 (2013). https://doi.org/10.1007/s12562-013-0616-7

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  • DOI: https://doi.org/10.1007/s12562-013-0616-7

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