Abstract
Dynein motors are biologically important bio-nanomachines, and many atomic resolution structures of cytoplasmic dynein components from different organisms have been analyzed by X-ray crystallography, cryo-EM, and NMR spectroscopy. This review provides a historical perspective of structural studies of cytoplasmic and axonemal dynein including accessory proteins. We describe representative structural studies of every component of dynein and summarize them as a structural atlas that classifies the cytoplasmic and axonemal dyneins. Based on our review of all dynein structures in the Protein Data Bank, we raise two important points for understanding the two types of dynein motor and discuss the potential prospects of future structural studies.
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Acknowledgements
We thank the staff of the beamline BL44XU at SPring-8, Japan, for the help during data collection and Prof. Toshiki Yagi for providing the plasmid used in this study.
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This work was supported by a Grant-in-Aid for Scientific Research B (JP26291014) from the Japan Society for the Promotion of Science (JSPS) and JSPS Fellows Number JP17J07553.
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Akiyuki Toda declares that he has no conflict of interest. Hideaki Tanaka declares that he has no conflict of interest. Genji Kurisu declares that he has no conflict of interest.
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This article is part of a Special Issue on “Biomolecules to Bio-nanomachines - Fumio Arisaka 70th Birthday” edited by Damien Hall, Junichi Takagi, and Haruki Nakamura.
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Toda, A., Tanaka, H. & Kurisu, G. Structural atlas of dynein motors at atomic resolution. Biophys Rev 10, 677–686 (2018). https://doi.org/10.1007/s12551-018-0402-y
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DOI: https://doi.org/10.1007/s12551-018-0402-y