Abstract
Sedimentation velocity analytical ultracentrifugation (SV-AUC) coupled with direct computational fitting of the observed concentration profiles (sedimentating boundary) have been developed and widely used for the characterization of macromolecules and nanoparticles in solution. In particular, size distribution analysis by SV-AUC has become a reliable and essential approach for the characterization of biopharmaceuticals including therapeutic antibodies. In this review, we describe the importance and advantages of SV-AUC for studying biopharmaceuticals, with an emphasis on strategies for sample preparation, data acquisition, and data analysis. Recent discoveries enabled by AUC with a fluorescence detection system and potential future applications are also discussed.
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This work was partly supported by the MEXT/JSPS Grants in Aid for Scientific Research (15 K14457, 17H03975) and by grant-in-aid from the Research on Development of New Drugs of AMED.
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Susumu Uchiyama declares that he has no conflicts of interest. Masanori Noda declares that he has no conflicts of interest. Elena Krayukhina declares that she has no conflicts of interest.
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This article is part of a Special Issue on ‘Biomolecules to Bio-nanomachines - Fumio Arisaka 70th Birthday’ edited by Damien Hall, Junichi Takagi and Haruki Nakamura.
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Uchiyama, S., Noda, M. & Krayukhina, E. Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies. Biophys Rev 10, 259–269 (2018). https://doi.org/10.1007/s12551-017-0374-3
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DOI: https://doi.org/10.1007/s12551-017-0374-3