Biophysical Reviews

, Volume 10, Issue 2, pp 141–144 | Cite as

Most of it started with T4 phage and was then taken over

  • Shigeki Takeda
Brief Communication


Professor Fumio Arisaka is one of the famous leaders in bacteriophage research, especially in the areas of protein biophysics and structural biology. Autonomous phage morphogenesis is a self-assembly process controlled by subunit–subunit interaction. Under this principle, Fumio has studied T4 tail assembly and morphology. He has also contributed structural information about T4 phage through a combination of X-ray structural analysis and three-dimensional image reconstruction using cryo-electron microscopy. Most of the development of ultracentrifugation applications for molecular assembly and phage morphogenesis research was also performed in Fumio’s laboratory. Fumio is a pioneer of supramolecular protein assembly study, and his science continues in the research work of the approximately 150 people who had attended his final lecture at the Tokyo Institute of Technology.


Bacteriophage T4 Self-assembly Protein-protein interaction 


Compliance with ethical standards

Conflict of interest

Shigeki Takeda declares that he has no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Akhter T, Zhao L, Kohda A, Mio K, Kanamaru S, Arisaka F (2007) The neck of bacteriophage T4 is a ring-like structure formed by a hetero-oligomer of gp13 and gp14. Biochim Biophys Acta 1774:1036–1043CrossRefPubMedGoogle Scholar
  2. Aksyuk AA, Leiman PG, Kurochkina LP, Shneider MM, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG (2009) The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. EMBO J 28:821–829CrossRefPubMedPubMedCentralGoogle Scholar
  3. Arisaka F, Tschopp J, van Driel R, Engel J (1979) Reassembly of the bacteriophage T4 tail from the core-baseplate and the monomeric sheath protein P18: a co-operative association process. J Mol Biol 132:369–386CrossRefPubMedGoogle Scholar
  4. Arisaka F, Engel J, Klump H (1981) Contraction and dissociation of the bacteriophage T4 tail sheath induced by heat and urea. Prog Clin Biol Res 64:365–379PubMedGoogle Scholar
  5. Arisaka F, Ishimoto L, Kassavetis G, Kumazaki T, Ishii S (1988) Nucleotide sequence of the tail tube structural gene of bacteriophage T4. J Virol 62:882–886PubMedPubMedCentralGoogle Scholar
  6. Arisaka F, Takeda S, Funane K, Nishijima N, Ishii S (1990) Structural studies of the contractile tail sheath protein of bacteriophage T4. 2. Structural analyses of the tail sheath protein, gp18, by limited proteolysis, immunoblotting and immunoelectron microscopy. Biochemistry 29:5057–5062CrossRefPubMedGoogle Scholar
  7. Arisaka F, Yap ML, Kanamaru S, Rossmann MG (2016) Molecular assembly and structure of the bacteriophage T4 tail. Biophys Rev 8:385–396CrossRefPubMedPubMedCentralGoogle Scholar
  8. Belnap DM, Kumar A, Folk JT, Smith TJ, Baker TS (1999) Low-resolution density maps from atomic models: how stepping “back” can be a step “forward”. J Struct Biol 125:166–175CrossRefPubMedGoogle Scholar
  9. Brenner S, Horne RW (1959) A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta 34:103–110CrossRefPubMedGoogle Scholar
  10. Coombs DH, Arisaka F (1994) T4 tail structure and function. In: Karam JD (ed) Molecular biology of bacteriophage T4. American Society for Microbiology, Washington DC, pp 259–281Google Scholar
  11. Crick FH, Barnett L, Brenner S, Watts-Tobin RJ (1961) General nature of the genetic code for proteins. Nature 192:1227–1232CrossRefPubMedGoogle Scholar
  12. Eiserling FA, Black LW (1994) Pathways in T4 morphogenesis. In: Karam JD (ed) Molecular biology of bacteriophage T4. American Society for Microbiology, Washington DC, pp 209–212Google Scholar
  13. Harada K, Yamashita E, Nakagawa A, Miyafusa T, Tsumoto K, Ueno T, Toyama Y, Takeda S (2013) Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion. Biochim Biophys Acta 1834:284–291CrossRefPubMedGoogle Scholar
  14. Hershey AD, Chase M (1952) Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol 36:39–56CrossRefPubMedPubMedCentralGoogle Scholar
  15. Kanamaru S, Leiman PG, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Arisaka F, Rossmann MG (2002) Structure of the cell-puncturing device of bacteriophage T4. Nature 415:553–557CrossRefPubMedGoogle Scholar
  16. Kikuchi Y, King J (1975) Genetic control of bacteriophage T4 baseplate morphogenesis. I. Sequential assembly of the major precursor, in vivo and in vitro. J Mol Biol 99:645–672CrossRefPubMedGoogle Scholar
  17. King J (1971) Bacteriophage T4 tail assembly: four steps in core formation. J Mol Biol 58:693–709CrossRefPubMedGoogle Scholar
  18. Kitazawa D, Takeda S, Kageyama Y, Tomihara M, Fukada H (2005) Expression and characterization of a baseplate protein for bacteriophage Mu, gp44. J Biochem 137:601–606CrossRefPubMedGoogle Scholar
  19. Kondou Y, Kitazawa D, Takeda S, Tsuchiya Y, Yamashita E, Mizuguchi M, Kawano K, Tsukihara T (2005) Structure of the central hub of bacteriophage Mu baseplate determined by X-ray crystallography of gp44. J Mol Biol 352:976–985CrossRefPubMedGoogle Scholar
  20. Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG (2003) Three-dimensional structure of bacteriophage T4 baseplate. Nat Struct Biol 10:688–693CrossRefPubMedGoogle Scholar
  21. Kostyuchenko VA, Chipman PR, Leiman PG, Arisaka F, Mesyanzhinov VV, Rossmann MG (2005) The tail structure of bacteriophage T4 and its mechanism of contraction. Nat Struct Mol Biol 12:810–813CrossRefPubMedGoogle Scholar
  22. Leiman PG, Chipman PR, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG (2004) Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Cell 118:419–429CrossRefPubMedGoogle Scholar
  23. Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG (2010) Morphogenesis of the T4 tail and tail fibers. Virol J 7:355–382CrossRefPubMedPubMedCentralGoogle Scholar
  24. Nakagawa H, Arisaka F, Ishii S (1985) Isolation and characterization of the bacteriophage T4 tail-associated lysozyme. J Virol 54:460–466PubMedPubMedCentralGoogle Scholar
  25. Suzuki H, Yamada S, Toyama Y, Takeda S (2010) The C-terminal domain is sufficient for host-binding activity of the Mu phage tail-spike protein. Biochim Biophys Acta 1804:1738–1742CrossRefPubMedGoogle Scholar
  26. Takeda S, Arisaka F, Ishii S, Kyogoku Y (1990) Structural studies of the contractile tail sheath protein of bacteriophage T4. 1. Conformational change of the tail sheath upon contraction as probed by differential chemical modification. Biochemistry 29:5050–5056CrossRefPubMedGoogle Scholar
  27. Takeda S, Sasaki T, Ritani A, Howe MM, Arisaka F (1998a) Discovery of the tail tube gene of bacteriophage Mu and sequence analysis of the sheath and tube genes. Biochim Biophys Acta 1399:88–92CrossRefPubMedGoogle Scholar
  28. Takeda S, Hoshida K, Arisaka F (1998b) Mapping of functional sites on the primary structure of the tail lysozyme of bacteriophage T4 by mutational analysis. Biochim Biophys Acta 1384:243–252CrossRefPubMedGoogle Scholar
  29. Takeda S, Suzuki M, Yamada T, Kageyama M, Arisaka F (2004) Mapping of functional sites on the primary structure of the contractile tail sheath protein of bacteriophage T4 by mutation analysis. Biochim Biophys Acta 1699:163–171CrossRefPubMedGoogle Scholar
  30. Taylor NM, Prokhorov NS, Guerrero-Ferreira RC, Shneider MM, Browning C, Goldie KN, Stahlberg H, Leiman PG (2016) Structure of the T4 baseplate and its function in triggering sheath contraction. Nature 533:346–352CrossRefPubMedGoogle Scholar
  31. Tschopp J, Arisaka F, van Driel R, Engel J (1979) Purification, characterization and reassembly of the bacteriophage T4D tail sheath protein P18. J Mol Biol 128:247–258CrossRefPubMedGoogle Scholar
  32. Yap ML, Mio K, Leiman PG, Kanamaru S, Arisaka F (2010) The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro. J Mol Biol 395:349–360CrossRefPubMedGoogle Scholar

Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Faculty of Science and Technology, Division of Molecular ScienceGunma UniversityKiryuJapan

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