Mycotoxin Research

, Volume 34, Issue 2, pp 91–97 | Cite as

Multimycotoxin analysis of South African Aspergillus clavatus isolates

Original Article
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Abstract

Aspergillus clavatus poisoning is a neuromycotoxicosis of ruminants that occurs sporadically across the world after ingestion of infected feedstuffs. Although various toxic metabolites are synthesized by the fungus, it is not clear which specific or group of mycotoxins induces the syndrome. A. clavatus isolates were deposited in the culture collection of the Biosystematics Division, Plant Protection Research Institute, Agricultural Research Council during incidences of livestock poisoning (1988–2016). Six isolates were still viable and these plus three other South African isolates that were also previously deposited in the collection were positively identified as A. clavatus based on morphology and ß-tubulin sequence data. The cultures were screened for multiple mycotoxins using a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method. Twelve A. clavatus metabolites were detected. The concentrations of the tremorgenic mycotoxins (i.e., tryptoquivaline A and its related metabolites deoxytryptoquivaline A and deoxynortryptoquivaline) were higher than patulin and cytochalasin E. Livestock owners should not feed A. clavatus-infected material to ruminants as all the South African A. clavatus isolates synthesized the same compounds when cultured under similar conditions.

Keywords

Aspergillus clavatus Cytochalasin E Patulin Tremorgenic Tryptoquivaline 

Notes

Acknowledgements

The authors would also like to express their gratitude to Marie Smith, Stats4science, who performed the statistical analysis and Danielle Henn who assisted with the drawing of the chemical structures.

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Society for Mycotoxin Research and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Paraclinical Sciences, Faculty of Veterinary ScienceUniversity of PretoriaOnderstepoortSouth Africa
  2. 2.Biosystematics DivisionAgricultural Research Council - Vegetable and Ornamental PlantsPretoriaSouth Africa
  3. 3.Centre for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln)University of Natural Resources and Life SciencesTullnAustria

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