Two-Wavelength, Photo-Initiation and Photo-Inhibition Competing for Selective Photo-Patterning of Hydrogel Porous Microstructures
Ever since its development, tissue engineering has played a significant role in the medical arena with an ever-growing demand for various tissue donations. One crucial factor in conducting in vitro tissue engineering study is the construction of a desirable artificial three-dimensional (3D) hydrogel tissue scaffold to act as the extracellular matrix (ECM), meeting the complex requirements for specific cell cultures. Existing hydrogel scaffold fabrication techniques and systems utilized in constructing ECM are either twodimensionally limiting, hard to control the pattern morphologies or expensive and time consuming. In the present study, we introduce a simple, inexpensive method for selective patterning 3D porous microstructures. This technique-'two wavelength photo-initiation and photo inhibition competes’ is an extension of conventional photo-patterning method. Integrating with shadow mask, photo inhibition radicals were introduced to couple with the polymerization chains and terminate the photo crosslinking behavior at designed region, making 3D selectively patterning hydrogel feasible. High aspect ratio ridge with selectively inhibited porous structures and selectively patterned micro pillar were fabricated using this method within 1 minute. The in vitro cell test results indicate the patterned structures' good biocompatibility.
KeywordsHydrogel Microstructures Photo-inhibition Photo-initiation Photo-patterning
Unable to display preview. Download preview PDF.
- 1.Lanza, R., Langer, R., and Vacanti, J. P., “Principles of Tissue Engineering,” Elsevier, 1997.Google Scholar
- 11.Gross, B. C., Erkal, J. L., Lockwood, S. Y., Chen, C., and Spence, D. M., “Evaluation of 3D Printing and Its Potential Impact on Biotechnology and the Chemical Sciences,” ACS Publications, vol. 86, no. 7, pp. 3240–3253, 2014.Google Scholar
- 14.Woodfield, T., Guggenheim, M., Von Rechenberg, B., Riesle, J., Van Blitterswijk, C., and Wedler, V., “Rapid Prototyping of Anatomically Shaped, Tissue-Engineered Implants for Restoring Congruent Articulating Surfaces in Small Joints,” Cell Proliferation, vol. 42, no. 4, pp. 485–497, 2009.CrossRefGoogle Scholar
- 15.Mihaylova, E., “Holography-Basic Principles and Contemporary Applications,” InTech, pp. 45–47, 2013.Google Scholar
- 16.Lu, Y. and Chen, S., “Projection Printing of 3-Dimensional Tissue Scaffolds,” in: Computer-Aided Tissue Engineering, Liebschner, M., (Eds.), Springer, vol. 868, pp. 289–302, 2012.Google Scholar
- 17.Pfister, A., Landers, R., Laib, A., Hübner, U., Schmelzeisen, R., and Mülhaupt, R., “Biofunctional Rapid Prototyping for Tissue-Engineering Applications: 3D Bioplotting Versus 3D Printing,” Journal of Polymer Science Part A: Polymer Chemistry, vol. 42, no. 3, pp. 624–638, 2004.CrossRefGoogle Scholar
- 18.Chua, C. K., Leong, K. F., and Lim, C. S., “Rapid Prototyping: Principles and Applications,” World Scientific, 2010.Google Scholar
- 21.O'Neill, P., Ben Azouz, A., Vázquez, M., Liu, J., Marczak, S., et al., “Advances in Three-Dimensional Rapid Prototyping of Microfluidic Devices for Biological Applications,” Biomicrofluidics, Vol. 8, No. 5, Paper No. 052112, 2014.Google Scholar
- 22.Hong, M.-P., Kim, W.-S., Sung, J.-H., Kim, D.-H., Bae, K.-M., and Kim, Y.-S., “High-Performance Eco-Friendly Trimming Die Manufacturing Using Heterogeneous Material Additive Manufacturing Technologies,” International Journal of Precision Engineering and Manufacturing-Green Technology, vol. 5, no. 1, pp. 133–142, 2018.CrossRefGoogle Scholar
- 26.Xu, D., Chen, K. P., Ohlinger, K., and Lin, Y., “Nanoimprinting Lithography of a Two-Layer Phase Mask for Three-Dimensional Photonic Structure Holographic Fabrications Via Single Exposure,” Nanotechnology, Vol. 22, No. 3, Paper No. 035303, 2010.Google Scholar
- 27.Zhu, S., Fu, Y., and Hou, J., “Lithography: Principles, Processes and Materials,” in: Nova Science Publishers, Hennessy, T. C., (Ed.), pp. 119–132, 2012.Google Scholar
- 29.Park, S.-Y., Lee, J.-S., Choi, B.-H., Ahn, I. H., and Moon, S. K., “Modeling and Observation of Compressive Behaviors of Closed Celullar Structures Using Central Voronoi Tessellation Concepts,” International Journal of Precision Engineering and Manufacturing, vol. 16, no. 12, pp. 2459–2465, 2015.CrossRefGoogle Scholar