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Comparative Assessment of the Ability of Dual-Pore Structure and Hydroxyapatite to Enhance the Proliferation of Osteoblast-Like Cells in Well-Interconnected Scaffolds

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Abstract

In this study, to compare the relative abilities of dual-pore structure and hydroxyapatite to enhance the proliferation of osteoblastlike cell in well-interconnected scaffolds, several types of scaffolds were fabricated using combined SLUP (Salt-Leaching Using Powder) and WNM (Wire-Network Molding) techniques: well-interconnected dual-pore scaffolds with hydroxyapatite particles, wellinterconnected dual-pore scaffolds without hydroxyapatite particles, and single-pore scaffolds with hydroxyapatite particles. To assess the characteristics of the fabricated scaffolds, their morphology, compressive modulus, water absorption, and in-vitro cell activity were measured. Consequently, it was found that while the hydroxyapatite (which is hydrophilic) provides some advantage for cell attachment, the cell attachment in the dual-pore scaffold with hydroxyapatite particles was similar to that of the dual-pore scaffold without hydroxyapatite particles. Moreover, regarding cell proliferation, we verified that the effect of the dual-pore structure was dominant compared with the existence of hydroxyapatite particles and co-existence of dual-pore structure/hydroxyapatite particles. However, the cell vitality of the dual-pore scaffold with hydroxyapatite particles was higher than that of the dual-pore scaffold without hydroxyapatite particles because of ions released by the hydroxyapatite particles.

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Authors and Affiliations

  1. Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University, 460, Iksan-daero, Iksan-si, Jeollabuk-do, 54538, Republic of Korea

    Yong Sang Cho, Joon Sup Lee & Se-Hwan Lee

  2. Department of Mechanical Design Engineering, College of Engineering, Wonkwang University, 460, Iksan-daero, Iksan-si, Jeollabuk-do, 54538, Republic of Korea

    Young-Sam Cho

  3. Department of Orthopedics, Daejeon St. Mary’s Hospital, Catholic University of Korea, 64, Daeheung-ro, Jung-gu, Daejeon, 34943, Republic of Korea

    Myoung Wha Hong & Young Yul Kim

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  1. Yong Sang Cho
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  2. Joon Sup Lee
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Correspondence to Young Yul Kim or Young-Sam Cho.

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Cho, Y.S., Lee, J.S., Hong, M.W. et al. Comparative Assessment of the Ability of Dual-Pore Structure and Hydroxyapatite to Enhance the Proliferation of Osteoblast-Like Cells in Well-Interconnected Scaffolds. Int. J. Precis. Eng. Manuf. 19, 605–612 (2018). https://doi.org/10.1007/s12541-018-0073-4

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  • DOI: https://doi.org/10.1007/s12541-018-0073-4

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