Skip to main content
SpringerLink
Log in

Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing

  • Original Research Article
  • Published:
Interdisciplinary Sciences: Computational Life Sciences Aims and scope Submit manuscript

Abstract

16S rDNA sequencing which has gained wide popularity amongst microbiologists for the molecular characterization and identification of newly discovered isolates provides accurate identification of isolates down to the level of sub-species (strain). Its most important advantage over the traditional biochemical characterization methods is that it can provide an accurate identification of strains with atypical phenotypic characters as well. The following work is an application of 16S rRNA gene sequencing approach to identify a novel species of Probiotic Lactobacillus acidophilus. The sample was collected from pond water samples of rural and urban areas of Krishna district, Vijayawada, Andhra Pradesh, India. Subsequently, the sample was serially diluted and the aliquots were incubated for a suitable time period following which the suspected colony was subjected to 16S rDNA sequencing. The sequence aligned against other species was concluded to be a novel, Probiotic L. acidophilus bacteria, further which were named L. acidophilus strain EMBS081 & EMBS082. After the sequence characterization, the isolate was deposited in GenBank Database, maintained by the National Centre for Biotechnology Information NCBI. The sequence can also be retrieve from EMBL and DDBJ repositories with accession numbers JX255677 and KC150145.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Macfarlane GT, Cummings JH (1999) Probiotics and prebiotics: can regulating the activities of intestinal bacteria benefit health. West J Med 171(3):187–191

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Verschuere L, Rombaut G, Sorgeloos P (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64(4):655–671

    Article  CAS  Google Scholar 

  3. Balcázar JL, de Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrell D, Múzquiz JL (2006) The role of probiotics in aquaculture. Vet Microbiol 114(3–4):173–186

    Article  Google Scholar 

  4. Ninawe AS, Selvin J (2009) Probiotics in shrimp aquaculture: avenues and challenges. Crit Rev Microbiol 35(1):43–66

    Article  CAS  Google Scholar 

  5. Gupta V, Garg R (2009) Probiotics. Indian J Med Microbiol 27(3):202–209

    Article  CAS  Google Scholar 

  6. Corthésy B, Gaskins HR, Mercenier A (2007) Cross-talk between probiotic bacteria and the host immune system. J Nutr 137(3 Suppl 2):781S–790S

    Article  Google Scholar 

  7. Van Gossum A, Dewit O, Louis E, de Hertogh G, Baert F, Fontaine F, DeVos M, Enslen M, Paintin M, Franchimont D (2007) Multicenter randomized-controlled clinical trial of probiotics (Lactobacillus johnsonii, LA1) on early endoscopic recurrence of Crohn’s disease after lleo-caecal resection. Inflamm Bowel Dis 13(2):135–142

    Article  Google Scholar 

  8. Hickson M, D’Souza AL, Muthu N, Rogers TR, Want S, Rajkumar C, Bulpitt CJ (2007) Use of probiotic Lactobacillus preparation to prevent diarrhoea associated with antibiotics: randomised double blind placebo controlled trial. BMJ 335(7610):80

    Article  Google Scholar 

  9. Lin YP, Thibodeaux CH, Peña JA, Ferry GD, Versalovic J (2008) Probiotic Lactobacillus reuteri suppress proinflammatory cytokines via c-Jun. Inflamm Bowel Dis 14(8):1068–1083

    Article  Google Scholar 

  10. Elguezabal N, Chamorro S, Molina E, Garrido JM, Izeta A, Rodrigo L (2012) Juste RA (2012) Lactase persistence, NOD2 status and Mycobacterium avium subsp. paratuberculosis infection associations to Inflammatory Bowel Disease. Gut Pathog 4:6

    Article  CAS  Google Scholar 

  11. Hempel S, Newberry SJ, Maher AR, Wang Z, Miles JN, Shanman R, Johnsen B, Shekelle PG (2012) Probiotics for the prevention and treatment of antibiotic-associated diarrhea: a systematic review and meta-analysis. JAMA 307(18):1959–1969

    Article  CAS  Google Scholar 

  12. Kesarcodi-Watson A, Heinrich KM, Lategan J, Gibson L (2008) Probiotics in aquaculture: the need, principles and mechanisms of action and screening processes. Aquaculture 274(2008):1–14

    Article  Google Scholar 

  13. Sahu MK, Swarnakumar NS, Sivakumar K, Thangaradjou T, Kannan L (2008) Probiotics in aquaculture: importance and future perspectives. Indian J Microbiol 48(3):299–308

    Article  Google Scholar 

  14. Liu KX, Zhu YG, Zhang J, Tao LL, Lee JW, Wang XD, Qu JM (2012) Probiotics’ effects on the incidence of nosocomial pneumonia in critically ill patients: a systematic review and meta-analysis. Critical Care 2012(16):R109

    Article  Google Scholar 

  15. Desfossés-Foucault É, Dussault-Lepage V, Le Boucher C, Savard P, LaPointe G, Roy D (2012) Assessment of probiotic viability during Cheddar cheese manufacture and ripening using propidium monoazide-PCR quantification. Front Microbiol 3:350

    Article  Google Scholar 

  16. Verna EC (2010) Use of probiotics in gastrointestinal disorders: What to recommend? Therap Adv Gastroenterol 3(5):307–319

    Article  Google Scholar 

  17. Neal-McKinney JM, Lu X, Duong T, Larson CL, Call DR, Shah DH, Konkel ME (2012) Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry. PLoS One 7(9):e43928

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India

    Harshpreet Chandok, Pratik Shah, Uday Raj Akare, Maliram Hindala, Sneha Singh Bhadoriya, G. V. Ravi, Varsha Sharma & Anuraj Nayarisseri

  2. Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016, India

    Srinivas Bandaru

  3. Department of Biotechnology, Sanghvi Institute of Management & Science, Indore, 453 331, India

    Pragya Rathore

Authors
  1. Harshpreet Chandok
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pratik Shah
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Uday Raj Akare
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maliram Hindala
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sneha Singh Bhadoriya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. V. Ravi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Varsha Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Srinivas Bandaru
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Pragya Rathore
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Anuraj Nayarisseri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anuraj Nayarisseri.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chandok, H., Shah, P., Akare, U.R. et al. Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing. Interdiscip Sci Comput Life Sci 7, 242–248 (2015). https://doi.org/10.1007/s12539-015-0002-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12539-015-0002-5

Keywords

Search

Navigation