Abstract
Purpose
Current approaches to in vitro maturation (IVM) may result in low efficiency and inadequate quality of the oocytes due to insufficient cytoplasmic maturation. Although positive effects of the cysteamine supplementation in IVM medium for oocyte nuclear maturation or male pronuclear formation have been confirmed, it is still controversial whether the cysteamine addition affects embryo development after IVM. We aimed here to confirm the effect of cysteamine addition into IVM medium for subsequent embryo development in vitro.
Methods
We administered the cysteamine to the IVM culture of rabbit immature oocytes at various concentrations and observed the developmental rate, speed to reach blastocyst stage and cell numbers at the blastocyst stage.
Results
Cysteamine supplementation improved developmental rate to blastocyst stage of the IVM oocytes. On the other hand, addition of glutathione (GSH) inhibitor buthionine sulfoximine inhibited GSH accumulation in the oocytes and subsequent embryo development to the blastocyst stage.
Conclusions
Controlling the GSH quantity of IVM oocytes may be an important factor for success of embryo development, and it is quite probable that a cysteamine supplementation can contribute to an increase of GSH content in oocyte.
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Acknowledgments
We gratefully acknowledge Ms. Naomi Backes Kamimura, Department of Biology-Oriented Science and Technology, Kinki University, for English editing.
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We have no conflict of interest.
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Izumi, H., Miyamoto, Y., Mori, T. et al. Cysteamine supplementation during in vitro maturation (IVM) of rabbit oocyte improves the developmental capacity after intracytoplasmic sperm injection. Reprod Med Biol 12, 179–185 (2013). https://doi.org/10.1007/s12522-013-0157-x
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DOI: https://doi.org/10.1007/s12522-013-0157-x