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Polymorphisms in the promoter region of IL10 gene are associated with virus etiology of infant bronchiolitis

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Abstract

Background

Bronchiolitis is the most common infection leading to hospitalization in infancy. Interleukin-10 (IL-10) is an anti-inflammatory cytokine, and in our previous study, IL10 gene rs1800896 (− 1082A/G) polymorphism was associated with viral etiology of infant bronchiolitis. The objective of this study was to evaluate the associations between IL10 single nucleotide polymorphisms (SNPs) at rs1800890 (− 3575A/T), rs1800871 (− 819C/T) or rs1800872 (− 592C/A) either alone or combined with the SNP at rs1800896 (− 1082G/A), and the etiology and severity of infant bronchiolitis.

Methods

Data on four IL10 SNPs were available from 135 full-term infants, hospitalized for bronchiolitis at age less than 6 months, and from 378 to 400 controls. Viral etiology was studied, and oxygen support, feeding support and the length of stay in hospital were recorded during bronchiolitis hospitalization.

Results

Infants with rhinovirus bronchiolitis had the IL10 rs1800890 variant AT or TT genotype less often (18.2%) than controls (63.3%, P = 0.03), and likewise, had the IL10 rs1800896 variant AG or GG genotype less often (27.3%) than controls (65.5%, P = 0.009). Twenty-eight infants with bronchiolitis had the variant–variant Grs1800896Trs1800890 haplotype, and none of them had rhinovirus infection. The IL10 rs1800871 or rs1800872 genotypes showed no associations with viruses. No association was found between any genotypes and bronchiolitis severity measures.

Conclusion

IL10 rs1800890 and rs1800896 polymorphisms differed between infants with rhinovirus bronchiolitis and controls, but not between infants with respiratory syncytial virus bronchiolitis and controls.

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Funding

This study was funded by the Tampere Tuberculosis Foundation (authors KN and MK).

Author information

Authors and Affiliations

  1. Seinäjoki Central Hospital, Hanneksenrinne 7, 60220, Seinäjoki, Finland

    Annukka Holster & Kirsi Nuolivirta

  2. Department of Medical Microbiology and Immunology, Turku University, Turku, Finland

    Johanna Teräsjärvi, Juho Vuononvirta & Qiushui He

  3. Tampere Center for Child Health Research, Tampere University and University Hospital, Tampere, Finland

    Petri Koponen, Merja Helminen & Matti Korppi

  4. Department of Pediatrics and Adolescent Medicine, Turku University Hospital and Child and Youth Research Institute, University of Turku, Turku, Finland

    Ville Peltola

  5. Department of Medical Microbiology, Capital Medical University, Beijing, China

    Qiushui He

Authors
  1. Annukka Holster
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  2. Johanna Teräsjärvi
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  3. Juho Vuononvirta
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  4. Petri Koponen
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  6. Merja Helminen
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  7. Qiushui He
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  8. Matti Korppi
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  9. Kirsi Nuolivirta
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Contributions

AH had responsibility for writing the manuscript. JT, JV and QH had responsibility for the genetic analyses and they participated in writing the manuscript. PK and MH participated in the protocol development and writing the manuscript. VP had responsibility for the STEPS study, enrolling the control patients and arranging the laboratory analyses. He also participated in writing the manuscript. MK and KN had responsibility for protocol development, patient screening, data analysis and writing the manuscript. All the authors have read and accepted the final manuscript.

Corresponding author

Correspondence to Kirsi Nuolivirta.

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of the Tampere University Hospital district, Tampere, Finland and with the 1964 WMA Helsinki Declaration. Before we enrolled the children we obtained informed parental consent, including the use of samples for genetic studies on bronchiolitis and asthma risk, both during hospitalization and at the control visit. The personal data of the study subjects were not given to the two laboratories that performed the genetic studies, the National Institute for Health and Welfare, Turku, Finland, and the Laboratory Center of Tampere University Hospital, Tampere, Finland. The STEPS study concerning the genetic control group of children was approved by the Ethics Committee of the Hospital District of Southwest Finland, Turku, Finland. A written, informed consent was obtained from the control children’s parents.

Conflict of interest

The authors declare that they have no conflict of interest. No financial or non-financial benefits have been received or will be received from any party related directly or indirectly to the subject of this article.

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Holster, A., Teräsjärvi, J., Vuononvirta, J. et al. Polymorphisms in the promoter region of IL10 gene are associated with virus etiology of infant bronchiolitis. World J Pediatr 14, 594–600 (2018). https://doi.org/10.1007/s12519-018-0161-7

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  • DOI: https://doi.org/10.1007/s12519-018-0161-7

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