Abstract
Enteric bacteria, many of which are opportunistic pathogens, were detected in groundwater from springs, wells, and a cave stream in the karst region of southwestern Illinois, and concentrations generally were very high in the springs and cave stream. The two main sources of bacterial contamination were determined to be domestic wastewater treatment discharge and livestock manure. The water chemistry in the springs and caves indicated substantial dilution of any wastewater discharge, but the dilution was not sufficient to lower bacteria concentrations to regulatory levels. High counts of enteric bacteria were found throughout the year, suggesting a continuous source of contamination, most likely domestic wastewater discharge. Although wells generally were less contaminated than springs, wells located in livestock areas usually were contaminated with enteric bacteria, and their water chemistry was indicative of contamination by animal waste.
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References
Burks BD, Minnis MM (1994) Onsite wastewater treatment systems. Hogarth House, Madison
Cason E, Greiman L, Reynolds D (1991) Bacterial species isolated from well water in southern Illinois. Dairy Food Environ Sanitation 11:645–649
Celico F, Varcamonti M, Guida M (2004) Influence of precipitation and soil on transport of fecal enterococci in fractured limestone aquifers. Appl Environ Microbiol 70:2843–2847
Clesceri LS, Greenburg AE, Trussel RR (1989) Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association, Washington, pp 9-1–9-280
Currens JC (2002) Changes in groundwater quality in a conduit-flow-dominated karst aquifer, following BMP implementation. Environ Geol 42:525–531
Freeman BA (1985) Burrow’s textbook of microbiology, 22nd edn. Saunders, Philadelphia
Geldreich EE (1996) Microbial quality of water supply in distribution systems. Lewis, New York
Greenberg AE, Trussell RR, Clesceri LS (1989) Standard methods for examination of water and wastewater, 16th edn. American Public Health Association, Washington, p 977
Hackley KC, Panno SV, Hwang HH et al (2008) Groundwater quality of springs and wells of the sinkhole plain in southwestern Illinois: Determination of the dominant sources of nitrate. Illinois State Geological Survey Circular 570, Champaign, IL
Harrison JJ, Turner RJ, Marques LLR (2005) Biofilms Am Sci 93:508–515
Herzog BL, Stiff BJ, Chenoweth CA (1994) Buried bedrock surface of Illinois. Illinois State Geological Survey, Champaign, Map 5
Hosmer DW Jr, Lemeshow S (2000) Applied logistic regression, 2nd edn. Wiley, New York
Hunter C, Perkins J, Tranter J (1999) Agricultural land-use effects on the indicator bacterial quality of an upland stream in the Derbyshire Peak District in the U.K. Water Res 33:3577–3586
Illinois Agricultural Statistics Service (2007) Illinois Agricultural Statistics Service annual summaries. Available via http://www.agstats.state.il.us/website/reports.htm
Illinois Environmental Protection Agency (1999) Illinois Environmental Protection Agency, Illinois Pollution Control Board. Title #35: Environmental Protection, Subtitle C: Water Pollution, Chapter I: Pollution Control Board, State of Illinois Rules and Regulations 302.209
Mahler BJ, Personné JC, Lods GF (2000) Transport of free and particulate-associated bacteria in karst. J Hydrol 238:179–193
Panno SV, Kelly WR (2004) Nitrate and herbicide loading in two groundwater basins of Illinois’ Sinkhole Plain. J Hydrol 290:229–242
Panno SV, Weibel CP, Heigold PC (1994) Formation of regolith-collapse sinkholes in southern Illinois: Interpretation and identification of associated buried cavities. Environ Geol 23:214–220
Panno SV, Krapac IG, Weibel CP et al (1996) Groundwater contamination in karst terrain of southwestern Illinois. Illinois State Geological Survey, Champaign, IL, Environment Geology Report EG 151
Panno SV, Weibel CP, Li WB (1997) Karst regions of Illinois. Illinois State Geological Survey, Champaign, IL, Open File Series 1997-2
Panno SV, Hackley KC, Hwang HH (2006a) Characterization and identification of Na-Cl sources in ground water. Ground Water 44:176–187
Panno SV, Kelly WR, Martinsek AT (2006b) Estimating background and threshold nitrate concentrations using probability graphs. Ground Water 44:697–709
Panno SV, Hackley KC, Kelly WR (2006c) Potential effects of recurrent low oxygen conditions on the Illinois Cave Amphipod. J Cave Karst Stud 68:55–63
Panno SV, Kelly WR, Hackley KC (2007) Chemical and bacterial quality of discharge from on-site aeration-type wastewater treatment systems. Ground Water Monitor Remed 27:71–76
Pasquarell GC, Boyer DG (1995) Agricultural impacts on bacterial water quality in karst groundwater. J Environ Qual 24:959–969
Personné JC, Poty F, Vaute L (1998) Survival, transport and dissemination of Escherichia coli and enterococci in a fissured environment Study of a flood in a karstic aquifer. J Appl Microbiol 84:431–438
Peterson EW, Davis RK, Orndorff HA (2000) 17 beta-estradiol as an indicator of animal waste contamination in mantled karst aquifers. J Environ Qual 29:826–834
Ryan M, Meiman J (1996) An examination of short-term variations in water quality at a karst spring in Kentucky. Ground Water 34:23–30
Thurman EM, Meyer M, Pomes M (1990) Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water. Anal Chem 62:2043–2048
White WB (1988) Geomorphology and hydrology of karst terrains. Oxford University Press, New York
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Kelly, W.R., Panno, S.V., Hackley, K.C. et al. Bacteria Contamination of Groundwater in a Mixed Land-Use Karst Region. Water Expo. Health 1, 69–78 (2009). https://doi.org/10.1007/s12403-009-0006-7
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DOI: https://doi.org/10.1007/s12403-009-0006-7