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Journal of Plant Biology

, Volume 61, Issue 2, pp 61–71 | Cite as

PsnERF75 Transcription Factor from Populus simonii × P. nigra Confers Salt Tolerance in Transgenic Arabidopsis

  • Shengji Wang
  • Boru Zhou
  • Wenjing Yao
  • Tingbo Jiang
Original Article
  • 102 Downloads

Abstract

ERF transcription factor involves in many aspects of plant development and response to both biotic and abiotic environmental stimuli. In this study, we provided evidence that PsnERF75 can improve the salt tolerance ability of transgenic Arabidopsis. RT-qPCR analysis described the spatio-temporal expression patterns of PsnERF75 in different poplar tissues. Under salt stress condition, root and leaf tissues were more sensitive than stem tissue. In addition, seed germination rates of PsnERF75 transgenic Arabidopsis were significantly enhanced. Growth status of PsnERF75 transgenic Arabidopsis seedlings was much better than that of the controls. Physiological and histochemical assays indicated PsnERF75 transgenic Arabidopsis have a greater capacity to eliminate reactive oxygen species and lighten the damages to plants than the controls. Using RNA-seq analysis, 100 DEGs that were significant differentially expressed between PsnERF75 transgenic Arabidopsis and the controls under salt stress conditions were identified. Seventeen upregulated and 10 down-regulated DEGs participate in plant salt stress response process. In addition, subcellular localization showed that PsnERF75 is a nuclear-localized protein. All of the results will provide novel insights into the functions of ERF75 in plants under abiotic stress condition.

Keywords

Abiotc stress ERF Populus simonii × P. nigra Plant physiology 

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Supplementary material

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Shengji Wang
    • 1
    • 2
  • Boru Zhou
    • 2
  • Wenjing Yao
    • 2
  • Tingbo Jiang
    • 2
  1. 1.College of ForestryShanxi Agricultural UniversityTaigu, ShanxiChina
  2. 2.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinChina

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