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Sugar Tech

pp 1–9 | Cite as

Transcriptome Reveals Differentially Expressed Genes in Saccharum spontaneum GX83-10 Leaf Under Drought Stress

  • Kai-Chao Wu
  • Li-Ping Wei
  • Cheng-Mei Huang
  • Yuan-Wen Wei
  • Hui-Qing Cao
  • Lin Xu
  • Hai-Bin Luo
  • Sheng-Li Jiang
  • Zhi-Nian Deng
  • Yang-Rui Li
Research Article

Abstract

Saccharum spontaneum is the most important and widely used wild germplasm in sugarcane resistance breeding, which can improve sugarcane resistance and ratoon capacity. In order to further exploit and utilize the resistance genes in S. spontaneum, we used a new generation of sequencing technology Illumina HiSeq high-throughput platform to analyze the expression profile of transcriptome genes in S. spontaneum GX83-10 leaves under normal watering (SS_CK) and drought stress (SS_T). The sequencing data were assembled by de novo, and functional annotation, differential gene screening and enrichment analysis were done. The results showed that 54499640 and 56440692 clean reads were obtained from the drought stress group and the control group, respectively. A total of 88941 unigenes and 1325 significantly differentially expressed genes (DEGs) were obtained in this study. The 125 functional gene groups were enriched by GO enrichment analysis. Five metabolic pathways were obtained by KEGG enrichment analysis, namely ascorbate and aldarate metabolism, plant hormone signal transduction, carotenoid biosynthesis, starch and sucrose metabolism, and phenylpropanoid biosynthesis. Seven significantly up-regulated genes were selected to conduct qRT-PCR analysis, and the results confirmed that all the seven genes were significantly up-regulated at varying degrees under drought stress. This study revealed the molecular mechanism of S. spontaneum leaf in response to drought stress and provided a reference for researches on related key genes which would be beneficial to breed new drought-resistant sugarcane varieties.

Keywords

Saccharum spontaneum Transcriptome reveals Differentially expressed genes Drought stress 

Notes

Funding

This work was financially supported by the National Science Foundation of China (31400281, 31460093, 31760415), Guangxi Natural Science Fund (2014GXNSFAA118128), Special Fund for Bagui Scholars (2013-03), Fund for Guangxi Innovation Teams of Modern Agriculture Technology (gjnytxgxcxtd-03-01) and Fund of Guangxi Academy of Agricultural Sciences (2015JZ11).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Sugar Research & Promotion 2018

Authors and Affiliations

  1. 1.Sugarcane Research InstituteGuangxi Academy of Agricultural SciencesNanningPeople’s Republic of China
  2. 2.Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi)Ministry of AgricultureNanningPeople’s Republic of China
  3. 3.Guangxi Key Laboratory of Sugarcane Genetic ImprovementNanningPeople’s Republic of China
  4. 4.Biotechnology Research InstituteGuangxi Academy of Agricultural SciencesNanningPeople’s Republic of China
  5. 5.Guangxi Crop Genetic Improvement and Biotechnology LaboratoryGuangxi Academy of Agricultural SciencesNanningPeople’s Republic of China

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