Advertisement

Sugar Tech

, Volume 20, Issue 2, pp 163–167 | Cite as

Production of Humic Acid by a Bacillus megaterium Strain Using Vinasse

  • Nan Li
  • Zhi-Nian Deng
  • Yuan-Wen Wei
  • Hui-Qing Cao
  • Yang-Rui Li
Research Article

Abstract

Humic acid can be produced by a Bacillus megaterium strain, AS019, using vinasse (sugarcane mill wastewater) at an optimal concentration of 12 °Bx. Using single factor and orthogonal experiments, the optimal culture conditions were determined: optimum inoculum size, 15%; initial pH, 6.0; culture time, 8 days; rotation speed, 180 rpm; and temperature, 28 °C. Under the optimal conditions, the humic acid production rate for the strain was 49.50 g/L. The results of characterization and infrared spectral analysis revealed that the produced humic acid contained a variety of reactive functional groups.

Keywords

Bacillus megaterium Vinasse Humic acid Fermentation condition Infrared spectrum 

Notes

Funding

This work was supported in part by Guangxi Special Funds for Bagui Scholars (2013-03), Project of Guangxi Sugarcane Innovation team of National Modern Agriculture Industry Technology System (gjnytxgxcxtd-03).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Fein, J.B., J.F. Boily, K. Guclu, and E. Kaulbach. 1999. Experimental study of humic acid adsorption onto bacteria and Al-oxide mineral surfaces. Chemical Geology 162: 33–451.CrossRefGoogle Scholar
  2. Feng, Y.Q. 2005. Developmental direction of humic acid fertilizer in China and its applicable example. Chemical Fertilizer Design 43: 56–61.Google Scholar
  3. Guan, P.F. 2005. The application of humic acid and humic fertilizer. Jilin Vegetables 5: 29–30.Google Scholar
  4. Guo, B., and C.X. Zhu. 2005. Bio-active humic acid in environment remediation. Humic Acid 5: 3–8.Google Scholar
  5. He, L.Q. 1999. Humic acid of research and application with biological technology, 115–164. Beijing: Chemical Industry Press.Google Scholar
  6. Huang, J.F., Y.L. Zhao, J.X. Zhao, J.F. Jiao, and T.Y. Li. 2007. The extraction of humic acid and its component content determination. Sichuan Animal and Veterinary Sciences 5: 27–28.Google Scholar
  7. Jesenak, K. 1982. The isolation of humic acid by means of extraction with 1 M sodium hydroxide solution. Acta Fac Rerum Nat Univ Comenianae Chim 30: 113–118.Google Scholar
  8. Jiang, P.W., Z.J. Ma, and Z.P. Li. 2009. The technology of extraction of humic acid from lignite and its status in application research. China Mining Magazine 18: 356–359.Google Scholar
  9. Khan, S.U. 1971. Distributio and characteristics of organic matter extracted from the black solonetzic and black chernozemic soils of Alberta: the humic acid fraction. Soil Science 112: 401–408.CrossRefGoogle Scholar
  10. Li, X.W., J.Y. Jin, and X.K. Ren. 2009. Application of humic fertilizer. Modernizing Agriculture 3: 30–31.Google Scholar
  11. Liu, K.D. 1999. Status and prospect of application field of humic acid. Fine and Specialty Chemicals 20: 13–14.Google Scholar
  12. Luan, J. 1995. The design and optimization method in modern experiment, 191–2011. Shanghai: Shanghai Jiaotong University Press.Google Scholar
  13. Ranjbar, H., R. Shahryari, and V. Mollasadeghi. 2012. Production of wheat seeds with presence of humic fertilizer after anthesis drought condition. African Journal of Agricultural Research 7: 307–310.Google Scholar
  14. Shahryari, R., A. Gadimov, E. Gurbanov, M. Valizadeh, and V. Mollasadeghi. 2011. Wheat genotypes response to terminal drought at presence of a humic fertilizer using stress tolerance. Advances in Environmental Biology 5: 166–168.Google Scholar
  15. Shan, J.J., C.S. Zhang, and J.C. Zhang. 2005. Exploiting new resource of BFA by bio-technique. Humic Acid 2: 1–3.Google Scholar
  16. Sposito, G., K.M. Holtzclaw, and J. Baham. 1976. Analytical properties of the soluble, metal-complexing fractions in sludge-soil mixtures: II. Comparative structural chemistry of fulvic acid. Soil Science Society of America Journal 40: 461–467.CrossRefGoogle Scholar
  17. Stevenson, F.J. 1994. Humus chemistry: genesis, composition, reactions. New York: Wiley.Google Scholar
  18. Wang, R.X. 2000. Mathematical statistics, 120–158. Xi’an: Xi’an Jiaotong University Press.Google Scholar
  19. Xu, E.G., and X.C. Zeng. 2004. Humic pesticide and its prospect of development. Humic Acid 2: 1–6.Google Scholar
  20. Xu, Q.X., X.T. Chen, Z.J. He, and Z. Liu. 2005. Study of processing paper mill disuse materiel-wheal straw with zymogen dosage. Tianjin Agricultural Sciences 11 (4): 18–19.Google Scholar
  21. Yang, M., H.B. Wang, and Y. Dai. 2002. Research on extractant of humic acid from humic soil and its infrared spectroscopy. Journal of Yunnan University for Nationalities (Natural Sciences Edition) 11: 573–574.Google Scholar
  22. Zeng, X.C. 2005. Humic acid and source security of production food. Humic Acid 4: 1–10.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 2017

Authors and Affiliations

  1. 1.College of Life Science and TechnologyGuangxi UniversityNanningChina
  2. 2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory, China Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Guangxi Key Laboratory of Sugarcane Genetic ImprovementGuangxi Academy of Agricultural SciencesNanningChina

Personalised recommendations