Sugar Tech

, Volume 20, Issue 2, pp 163–167 | Cite as

Production of Humic Acid by a Bacillus megaterium Strain Using Vinasse

  • Nan Li
  • Zhi-Nian Deng
  • Yuan-Wen Wei
  • Hui-Qing Cao
  • Yang-Rui Li
Research Article


Humic acid can be produced by a Bacillus megaterium strain, AS019, using vinasse (sugarcane mill wastewater) at an optimal concentration of 12 °Bx. Using single factor and orthogonal experiments, the optimal culture conditions were determined: optimum inoculum size, 15%; initial pH, 6.0; culture time, 8 days; rotation speed, 180 rpm; and temperature, 28 °C. Under the optimal conditions, the humic acid production rate for the strain was 49.50 g/L. The results of characterization and infrared spectral analysis revealed that the produced humic acid contained a variety of reactive functional groups.


Bacillus megaterium Vinasse Humic acid Fermentation condition Infrared spectrum 



This work was supported in part by Guangxi Special Funds for Bagui Scholars (2013-03), Project of Guangxi Sugarcane Innovation team of National Modern Agriculture Industry Technology System (gjnytxgxcxtd-03).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Society for Sugar Research & Promotion 2017

Authors and Affiliations

  1. 1.College of Life Science and TechnologyGuangxi UniversityNanningChina
  2. 2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory, China Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Guangxi Key Laboratory of Sugarcane Genetic ImprovementGuangxi Academy of Agricultural SciencesNanningChina

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