High-Density Genetic Map Construction in Sugar Beet (Beta vulgaris L.) by High-Throughput Technology
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A saturated genetic map can greatly promote the efficiency of gene mapping and marker-assisted selection (MAS) in plants. In the present study, we constructed an ultra-dense genetic map based on an F1 population derived from a cross between 3a (high-yield, low-sugar, sterile, monogerm, diploid line) and 3b (low-yield, high-sugar, pollinated, polyembryonic, diploid line) using Specific-Locus Amplified Fragment sequencing (SLAF-seq) technology. A total of 201.10 million high-quality pair-end reads were generated by Illumina high-throughput sequencing and a total of 171,637 SLAFs were developed, of which 48,478 were polymorphic. Finally, 3287 polymorphic SLAFs were mapped into an ultra-dense genetic map containing nine linkage groups (LGs). These markers had average depths of 105.84-, 104.16-, and 9.03-fold in the male parent, female parent, and progeny, respectively. The genetic map spanned 1554.64 cM with an average distance of 0.47 cM between adjacent markers. There were on an average 365 markers per linkage group and marker numbers ranging from 214 on LG 1 to 513 on LG 7. This genetic map will make great improvement for fine mapping QTLs and MAS in sugar beet.
KeywordsHigh-throughput technology Specific length amplified fragment Genetic map Sugar beet
This work was supported by the National Natural Science Foundation of China (31271780).
HZ and HW designed the study and performed the experiments; MW, YX and ZW performed the experiments, analyzed the data, and wrote the manuscript.
Compliance with Ethical Standards
Conflicts of interest
The authors declare no conflicts of interest.
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