Morphological and Physiological Responses of Sugar Beet to Alkaline Stress
- 63 Downloads
To explore the morphological and physiological responses of sugar beet to alkaline soils, four levels of alkaline (NaHCO3 + Na2CO3) concentration, including 0.0% (designated as A0.0%, control), 0.5% (designated as A0.5%), 0.7% (designated as A0.7%), and 0.9% (designated as A0.9%), were applied to sugar beet cultivars KWS0143 and Beta464 in a pot experiment in 2015 and 2016. The growth indexes, osmolyte content, and antioxidase activity of both cultivars were measured. Alkaline stress significantly decreased the emergence rate of both cultivars. Dry matter weight per plant and leaf chlorophyll content first increased but then decreased with increasing alkaline concentration at different stages in both years. The content of leaf osmolytes and the activity of antioxidases were enhanced at A0.5% and A0.7% but inhibited at A0.9%. These findings suggested that sugar beet adapted well to alkaline soil stress by osmotic adjustment and antioxidation and that its growth increased when stress was relatively low (A0.5% and A0.7%) at different stages. Moreover, proline content, CAT activity, and APX activity of KWS0143 under the same treatment were higher than those of Beta464. Our results confirmed that sugarbeet variety KWS0143 was showing tolerant behaviour to alkalinity.
KeywordsSugar beet Alkaline Osmotic adjustment Antioxidase
This study was funded by National Natural Science Foundation of China (Grant Number 31671622).
Compliance with Ethical Standards
Conflict of interest
Chunlei Zou, Limin Sang, and Caifeng Li have received research grants from Northeast Agricultural University. Zhijia Gai, from Heilongjiang Academy of Agricultural Sciences, participated in manuscript modification. Yubo Wang is a member of Northeast Agricultural University. The authors declare that they have no conflict of interest.
- Clifford, Sean C., Stefan K. Arndt, Janet E. Corlett, Sangeeta Joshi, Narendra Sankhla, Marianne Popp, and Hamlyn G. Jones. 1998. The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.). Journal of Experimental Botany 49(323): 967–977.CrossRefGoogle Scholar
- Flowers, Timothy J., and Timothy D. Colmer. 2008. Salinity tolerance in halophytes. Hoboken: Blackwell Publishing Ltd.Google Scholar
- Gong, Biao, Xiu Li, Sean Bloszies, Dan Wen, Shasha Sun, Min Wei, Yan Li, Fengjuan Yang, Qinghua Shi, and Xiufeng Wang. 2014. Sodic alkaline stress mitigation by interaction of nitric oxide and polyamines involves antioxidants and physiological strategies in Solanum lycopersicum. Free Radical Biology and Medicine 71(6): 36–48.CrossRefPubMedGoogle Scholar
- Li, Hesheng. 2003. Principle and technology of plant physiology and biochemistry experiment. Beijing: Higher Education Press.Google Scholar
- Liu, H., Q. Wang, M. Yu, Y. Zhang, Y. Wu, and H. Zhang. 2008. Transgenic salt-tolerant sugar beet (Beta vulgaris L.) constitutively expressing an Arabidopsis thaliana vacuolar Na/H antiporter gene, AtNHX3, accumulates more soluble sugar but less salt in storage roots. Plant Cell and Environment 31(9): 1325–1334.CrossRefGoogle Scholar
- Radić, Sandra, Petra Peharec Štefanić, Hrvoje Lepeduš, Vibor Roje, and Pevalek-Kozlina Branka. 2013. Salt tolerance of Centaurea ragusina L. is associated with efficient osmotic adjustment and increased antioxidative capacity. Environmental and Experimental Botany 87(1): 39–48.Google Scholar
- Xinjian, Yu., and Zhenqing Zhang. 1999. Modern experimental manual of plant physiology. Beijing: Science Press.Google Scholar
- Xu, Sheng, Jianlong Li, Xinquan Zhang, Hong Wei, and Langjun Cui. 2006. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress. Environmental and Experimental Botany 56(3): 274–285.CrossRefGoogle Scholar
- Zhang, Zhiliang. 2004. Experimental guide of plant physiology. Beijing: Higher Education Press.Google Scholar
- Zhang, Zhiliang, Qu Weijing, and Xiaofang Li. 2009. Experimental guide of plant physiology. Beijing: Higher Education Press.Google Scholar