Molecular Characterization of Pathogenicity Gene Homologs in Colletotrichum falcatum Causing Red Rot in Sugarcane
- 186 Downloads
Variation in Colletotrichum falcatum causing red rot disease of sugarcane is well established at cultural, pathogenic and molecular level. However, limited information is available on genes responsible for pathogen virulence and its pathogenesis. To understand virulence in C. falcatum, detailed studies were taken up using phylogenetically differentiated two isolates viz., Cf671 and Cf92020 vary in their virulence with 28 pathogenicity gene homologs. Important pathogenicity gene homologs amplified were PKS1, SCD1, THR1, Cap5, Cap20, MAF1, Pg1, PG2, Pel1, Pel2, SSD1, APH1, ArpA, ICL1, RGT2, RAC1, CreA, HXT1 and HXT3. Characterization of gene sequences of the selected pathogenicity genes from C. falcatum revealed both intra- and interspecific variation and comparison of genomic sources sequenced among two distinct isolates revealed a definite role of pathogenicity genes in C. falcatum pathogenesis. Differential expression of the pathogenicity gene homologs between isolates has been validated during host pathogen interaction.The present investigation identified the role of pathogenicity genes and their differential expression in C. falcatum pathogenesis for the first time.
KeywordsRed rot Colletotrichum falcatum Pathogenicity gene homologs Virulence
The authors are grateful to the Director, ICAR-Sugarcane Breeding Institute for providing facilities and constant encouragement.
This study was done as part of ICAR-SBI fund.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Dufresne, M., S. Perfect, A.L. Pellier, J.A. Bailey, and I. Langin. 2000. A GAL4-like protein is involved in the switch between biotrophic and necrotrophic phases of the infection process of Colletotrichum lindemuthianum on common bean. Plant Cell 12: 1579–1589.CrossRefPubMedPubMedCentralGoogle Scholar
- Keon, J.P.R., R.J.W. Byrde, and R. Cooper. 1987. Some aspects of fungal enzymes that degrade plant cell walls. In Fungal Infection of Plants, ed. G.I. Pegg, and P.G. Ayers, 133–157. Cambridge: Cambridge University Press.Google Scholar
- Malathi, P., R. Viswanathan, A. Ramesh Sundar, N. Prakasam, P. Padmanaban, R. Jothi, S.R. Renuka Devi, and M. Poongothai. 2010. Variability among Colletotrichum falcatum pathotypes used for screening red rot resistance in sugarcane. Sugar Cane International 28: 47–52.Google Scholar
- Malathi, P., R. Viswanathan, A. Ramesh Sundar, P. Padmanaban, N. Prakasam, D. Mohanraj, and R. Jothi. 2011. Phylogenetic analysis of Colletotrichum falcatum isolates causing red rot in sugarcane. Journal of Sugarcane Research 1: 69–74.Google Scholar
- Malathi, P., and R. Viswanathan. 2012. Functional analysis of pathogenicity gene homolog in Colletotrichum falcatum. In Proceedings at 3rd international conference on bio India ICBI-2012, held at Vivekanandha college of Engineering for women Elayampalayam, Tiruchengode, March 9–10, pp. 42.Google Scholar
- Manners, J.M., S.A. Stephenson, C. He, and D.J. Maclean. 2000. Gene transfer and expression in Colletotrichum gloeosporioides causing anthracnose on stylosanthes. In Colletotrichum host specificity, pathology and host-pathogen interaction, ed. D. Prusky, S. Freeman, and M.B. Dickman, 180–194. MN: APSPress St. Paul., USA.Google Scholar
- Srinivasan, K.V., and N.R. Bhatt. 1961. Criteria for grading resistance of sugarcane varieties to red rot (Glomeralla tucumanensis). Current Science 30 (11): 425.Google Scholar
- Takano, Y., Y. Kubo, C. Kawamura, T. Tsuge, and I. Furusawa. 1996. Complementation of an albino mutant (Pks–) of Colletotrichum lagenarium with Alternaria alternata ALM gene involved in melanin biosynthesis. (Abstr.) (In Japanese.) Annual Phytopathological Society of Japan 61: 617.Google Scholar
- Tanaka, S., K. Yamada, K. Yabumoto, S. Fuki, A. Huser, G. Tsuji, H. Koga, K. Dohi, M. Mori, R.T. Shiraishi O’Connell, and Y. Kubo. 2007. Saccharomyces cerevisiae SSD1 orthologues are essential for host infection by the ascomycete plant pathogens Colletotrichum lagenarium and Magnaporthe grisea. Molecular Microbiology 64: 1332–1349.CrossRefPubMedGoogle Scholar
- Viswanathan, R., P. Malathi, and P. Padmanaban. 2003. Variation in sugarcane red rot pathogen Colletotrichum falcatum Went. In Frontiers of Fungal Diversity in India, ed. G.P. Rao, C. Manoharachari, D.J. Bhat, R.C. Rajak, and T.N. Lakhanpal, 639–667. Lucknow: International Book Distributing Co.Google Scholar