Noninvasive estimation of quantitative myocardial blood flow with Tc-99m MIBI by a compartment model analysis in rat

  • Atsutaka Okizaki
  • Michihiro Nakayama
  • Kaori Nakajima
  • Osuke Fujimoto
  • Shinobu Oshikiri
  • Miho Koike-Satake
  • Yuto Nakahara
Original Article



We aimed to investigate the use of dynamic cardiac planar images to estimate myocardial blood flow (MBF) by a compartment model analysis using time-to-peak (TP) map and compared it by the microsphere technique in rat. Positron emission tomography is considered the gold standard method, but is not available everywhere. By contrast, although myocardial perfusion imaging (MPI) with single-photon tracers is more widely available, it may be difficult to obtain adequate region of interest (ROI) settings. We proposed using the TP map to set the ROI, and hypothesized that this method could facilitate the measurement of absolute MBF by MPI in rat.


Twenty-one normal rats were studied. Dynamic planar images with Tc-99m MIBI were obtained, and input function and cardiac ROIs were set using the obtained TP map. MBF was estimated by a one-compartment model analysis with the Renkin-Crone model and by the microsphere technique.


The MBFs from these two methods were significantly correlated. A negative proportional bias was observed, but no significant difference was observed between the mean MBFs calculated with each method.


MBF estimation by a compartment model analysis using TP map could facilitate absolute MBF measurement in rats.


TP map myocardial blood flow compartment model analysis Tc-99m sestamibi rats 



Coronary flow reserve


Myocardial blood flow


Positron emission tomography


Myocardial perfusion imaging

Tc-99m MIBI

Technetium-99m sestamibi




Single-photon emission computed tomography


Region of interest


Time-activity curve





We thank Hiroshi Kato and Kentaro Suzuki for animal surgery support, and Professor Koji Takahashi for allowing the use of all equipment.


O. Fujimoto, S. Oshikiri, M. Koike-Satake, and Y. Nakahara are employees of FujiFilm RI Pharma. No other potential conflict of interest relevant to this article was reported.

Author Contributions

AO wrote the main manuscript. AO and OF designed the study, and OF, SO, MK, and YN performed data acquisition and analysis, technical support. MN and KN performed statistical analysis.

Supplementary material

12350_2018_1274_MOESM1_ESM.pptx (276 kb)
Supplementary material 1 (PPTX 275 kb)


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Copyright information

© American Society of Nuclear Cardiology 2018

Authors and Affiliations

  • Atsutaka Okizaki
    • 1
  • Michihiro Nakayama
    • 1
  • Kaori Nakajima
    • 1
  • Osuke Fujimoto
    • 2
  • Shinobu Oshikiri
    • 2
  • Miho Koike-Satake
    • 2
  • Yuto Nakahara
    • 2
  1. 1.Department of RadiologyAsahikawa Medical UniversityAsahikawaJapan
  2. 2.FujiFilm RI Pharma Co., LtdTokyoJapan

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