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Measurement of myocardial fatty acid esterification using [1-11C]palmitate and PET: comparison with direct measurements of myocardial triglyceride synthesis

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Journal of Nuclear Cardiology Aims and scope

Abstract

Background

The purpose of the present study was to assess the accuracy of rates of myocardial fatty acid esterification (MFAE) obtained using positron emission tomography (PET).

Methods and results

Sixteen dogs were studied after an overnight fast (FAST), during a euglycemic hyperinsulinemic clamp (CLAMP), or during infusion of intralipid (IL) or IL plus dobutamine (IL/DOB). MFAE was quantified using [1-11C]palmitate and PET and compared to the rate of triglyceride (TG) synthesis measured using [1-13C]palmitate and tissue sampling. Plasma free fatty acid (FFA) concentration varied ~20-fold across groups, with this variation in FFA availability accompanied by a ~20-fold range in TG synthesis. MFAE varied ~12-fold across groups, and was significantly correlated with TG synthesis (R = 0.80, P < .001). MFAE, however, was 3- to 4-fold higher than TG synthesis in FAST, CLAMP, and IL, but only ~50% higher when cardiac work was increased in IL/DOB, suggesting that MFAE reflects, in part, the incorporation of label into amino acids via TCA cycle exchange reactions.

Conclusions

Changes in MFAE parallel changes in TG synthesis, at least in the basal state. Although the data need to be interpreted cautiously, such measurements should be useful for quantifying acute changes in FFA storage by the heart in various pathophysiological states.

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Acknowledgments

This research was supported by NIH grants HL-69100, RR-000954, DK-020579, and DK-056341.

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Authors and Affiliations

  1. Division of Radiological Sciences, Cardiovascular Imaging Laboratory, Edward Mallinckrodt Institute of Radiology, Washington University School of Medicine, 510 South Kingshighway Boulevard, St. Louis, MO, 63110, USA

    Andrew R. Coggan PhD, Zulfia Kisrieva-Ware MD, PhD, Carmen S. Dence PhD, Paul Eisenbeis BS, Robert J. Gropler MD & Pilar Herrero ME, MS

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  1. Andrew R. Coggan PhD
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  2. Zulfia Kisrieva-Ware MD, PhD
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  3. Carmen S. Dence PhD
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  5. Robert J. Gropler MD
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  6. Pilar Herrero ME, MS
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Correspondence to Andrew R. Coggan PhD.

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Coggan, A.R., Kisrieva-Ware, Z., Dence, C.S. et al. Measurement of myocardial fatty acid esterification using [1-11C]palmitate and PET: comparison with direct measurements of myocardial triglyceride synthesis. J. Nucl. Cardiol. 16, 562–570 (2009). https://doi.org/10.1007/s12350-009-9102-0

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  • DOI: https://doi.org/10.1007/s12350-009-9102-0

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