The Cerebellum

, Volume 17, Issue 5, pp 685–691 | Cite as

Centrosome Inheritance Does Not Regulate Cell Fate in Granule Neuron Progenitors of the Developing Cerebellum

  • Anindo Chatterjee
  • Kaviya Chinnappa
  • Narendrakumar Ramanan
  • Shyamala Mani
Short Report


An inherent asymmetry exists between the two centrosomes of a dividing cell. One centrosome is structurally more mature (mother centrosome) than the other (daughter centrosome). Post division, one daughter cell inherits the mother centrosome while the other daughter cell inherits the daughter centrosome. Remarkably, the kind of centrosome inherited is associated with cell fate in several developmental contexts such as in radial glial progenitors in the developing mouse cortex, Drosophila neuroblast divisions and in Drosophila male germline stem cells. However, the role of centrosome inheritance in granule neuron progenitors in the developing cerebellum has not been investigated. Here, we show that mother and daughter centrosomes do exist in these progenitors, and the amount of pericentriolar material (PCM) each centrosome possesses is different. However, we failed to observe any correlation between the fate adopted by the daughter cell and the nature of centrosome it inherited.


Mother centrosome Pericentriolar material (PCM) Cell fate Cerebellar granule neuron progenitors (CGNPs) 



The authors thank Parthiv Haldipur and Ajit Ray (National Brain Research Centre, India) for their inputs.

Funding Information

SM received funding from the Indo-French Centre for the Promotion of Advanced Research (CEFIPRA, grant no. 4903-02). KC received financial support from the Department of Biotechnology, Government of India (DBT Neurobiology Task Force, grant no. 0376). NR was supported by SwarnaJayanti Fellowship from the Department of Science and Technology, Government of India, and the DBT-IISc partnership program. AC received financial support from the Council of Scientific and Industrial Research (CSIR), Government of India.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Bornens M. The centrosome in cells and organisms. Science. 2012;335(6067):422–6.CrossRefPubMedGoogle Scholar
  2. 2.
    Yamashita YM, Fuller MT. Asymmetric centrosome behavior and the mechanisms of stem cell division. J Cell Biol. 2008;180(2):261–6.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Lambert JD, Nagy LM. Asymmetric inheritance of centrosomally localized mRNAs during embryonic cleavages. Nature. 2002;420(6916):682–6.CrossRefPubMedGoogle Scholar
  4. 4.
    Fuentealba LC, Eivers E, Geissert D, Taelman V, De Robertis EM. Asymmetric mitosis: unequal segregation of proteins destined for degradation. Proc Natl Acad Sci. 2008;105(22):7732–7.CrossRefPubMedGoogle Scholar
  5. 5.
    Habib SJ, Chen B, Tsai F, Anastassiadis K, Meyer T, Betzig E, et al. A localized Wnt signal orients asymmetric stem cell division in vitro. Science. 2013;1424(6126):1445–8.CrossRefGoogle Scholar
  6. 6.
    Yamashita YM, Mahowald AP, Perlin JR, Fuller MT. Asymmetric inheritance of mother versus daughter centrosome in stem cell division. Science. 2007;315(5811):518–21.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Wang X, Tsai J-W, Imai JH, Lian W-N, Vallee RB, Shi SH. Asymmetric centrosome inheritance maintains neural progenitors in the neocortex. Nature. 2009;461(7266):947–55.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Izumi H, Kaneko Y. Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells. Proc Natl Acad Sci. 2012;109(44):18048–53.CrossRefPubMedGoogle Scholar
  9. 9.
    Januschke J, Llamazares S, Reina J, Gonzalez C. Drosophila neuroblasts retain the daughter centrosome. Nat Commun. 2011;2:243.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Haldipur P, Sivaprakasm I, Periasamy P, Govindan S, Mani S. Asymmetric cell division of granule neuron progenitors in the external granule layer of the mouse cerebellum. Biol Open. 2015;4:865–72.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Ishikawa H, Kubo A, Tsukita S, Tsukita S. Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia. Nat Cell Biol. 2005;7(5):517–24.CrossRefPubMedGoogle Scholar
  12. 12.
    Mennella V, Agard DA, Huang B, Pelletier L. Amorphous no more: Subdiffraction view of the pericentriolar material architecture. Trends Cell Biol. 2014;24(3):188–97.CrossRefPubMedGoogle Scholar
  13. 13.
    Rebollo E, Sampaio P, Januschke J, Llamazares S, Varmark H, González C. Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells. Dev Cell. 2007;12(3):467–74.CrossRefPubMedGoogle Scholar
  14. 14.
    Lange BMH, Gull K. A molecular marker for centriole maturation in the mammalian cell cycle. J Cell Biol. 1995;130(4):919–27.CrossRefPubMedGoogle Scholar
  15. 15.
    Nakagawa Y, Yamane Y, Okanoue T, Tsukita S, Tsukita S. Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes. Mol Biol Cell. 2001;12(6):1687–97.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Anderson CT, Stearns T. Centriole age underlies asynchronous primary cilium growth in mammalian cells. Curr Biol. 2009;19(17):1498–502.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Donkor FF, Mönnich M, Czirr E, Hollemann T, Hoyer-Fender S. Outer dense fibre protein 2 (ODF2) is a self-interacting centrosomal protein with affinity for microtubules. J Cell Sci. 2004;117(20):4643–51.CrossRefPubMedGoogle Scholar
  18. 18.
    Sugioka K, Mizumoto K, Sawa H. Wnt regulates spindle asymmetry to generate asymmetric nuclear β-catenin in C. elegans. Cell. 2011;146(6):942–54.CrossRefPubMedGoogle Scholar
  19. 19.
    Tylkowski MA, Yang K, Hoyer-Fender S, Stoykova A. Pax6 controls centriole maturation in cortical progenitors through Odf2. Cell Mol Life Sci. 2015;72(9):1795–809.CrossRefPubMedGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre for NeuroscienceIndian Institute of ScienceBengaluruIndia
  2. 2.Curadev Pharma, Pvt. Ltd.NoidaIndia

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