ABA and BAP improve the accumulation of carbohydrates and alter carbon allocation in potato plants at elevated CO2

Abstract

Elevated CO2 interactions with other factors affects the plant performance. Regarding the differences between cultivars in response to CO2 concentrations, identifying the cultivars that better respond to such conditions would maximize their potential benefits. Increasing the ability of plants to benefit more from elevated CO2 levels alleviates the adverse effects of photoassimilate accumulation on photosynthesis and increases the productivity of plants. Despite its agronomic importance, there is no information about the interactive effects of elevated CO2 concentration and plant growth regulators (PGRs) on potato (Solanum tuberosum L.) plants. Hence, the physiological response and source-sink relationship of potato plants (cvs. Agria and Fontane) to combined application of CO2 levels (400 vs. 800 µmol mol−1) and plant growth regulators (PGR) [6-benzylaminopurine (BAP) + Abscisic acid (ABA)] were evaluated under a controlled environment. The results revealed a variation between the potato cultivars in response to a combination of PGRs and CO2 levels. Cultivars were different in leaf chlorophyll content; Agria had higher chlorophyll a, b, and total chlorophyll content by 23, 43, and 23%, respectively, compared with Fontane. The net photosynthetic rate was doubled at the elevated compared with the ambient CO2. In Agria, the ratio of leaf intercellular to ambient air CO2 concentrations [Ci:Ca] was declined in elevated-CO2-grown plants, which indicated the stomata would become more conservative at higher CO2 levels. On the other hand, the increased Ci:Ca in Fontane showed a stomatal acclimation to higher CO2 concentration. The higher leaf dark respiration of the elevated CO2-grown and BAP + ABA-treated plants was associated with a higher leaf soluble carbohydrates and starch content. Elevated CO2 and BAP + ABA shifted the dry matter partitioning to the belowground more than the above-media organs. The lower leaf soluble carbohydrate content and greater tuber yield in Fontane might indicate a more efficient photoassimilate translocation than Agria. The results highlighted positive synergic effects of the combined BAP + ABA and elevated CO2 on tuber yield and productivity of the potato plants.

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Abbreviations

CO2 :

Carbon dioxide

PGR:

Plant growth regulator

BAP:

6-Benzylaminopurine

ABA:

Abscisic acid

Ci/Ca :

The ratio of leaf intercellular to ambient air CO2 concentrations

SC:

Soluble carbohydrate content

ST:

Starch content

CK:

Cytokinins

GA:

Gibberellic acid

Chla:

Chlorophyll a

Chlb:

Chlorophyll b

Chl a + b:

Total chlorophyll

Chl a:B:

Chlorophyll a/b ratio

Car:

Carotenoids

Np:

Net photosynthetic rate

Rd :

Dark respiration

gs :

Stomatal conductance

Tr :

Transpiration rate

Ci :

Intercellular CO2

Φ:

Quantum yield of photosystem II

LDM:

Leaf dry matter

SDW:

Stem dry matter

RDM:

Root dry matter

TDM:

Tuber dry matter

Y:

Tuber yield

MTW:

Mean tuber weight

TN:

Tuber number

References

  1. Ahmadi-Lahijani MJ, Kafi M, Nezami A, Nabati J, Erwin J (2018) Effect of 6-benzylaminopurine and abscisic acid on gas exchange, biochemical traits, and minituber production of two potato cultivars (Solanum tuberosum L.). J Agric Sci Tech 20:129–139

    Google Scholar 

  2. Aien A, Pal M, Khetarpal S, Kumar Pandey S (2014) Impact of elevated atmospheric CO2 concentration on the growth, and yield in two potato cultivars. J Agric Sci Technol 16:1661–1670

    Google Scholar 

  3. Ainsworth EA, Rogers A (2007) The response of photosynthesis and stomatal conductance to rising [CO2]: mechanisms and environmental interactions. Plant Cell Environ 30:258–270

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  4. Aksenova N, Konstantinova T, Golyanovskaya S, Sergeeva L, Romanov G (2012) Hormonal regulation of tuber formation in potato plants. Russ J Plant Physiol 59:451–466

    CAS  Article  Google Scholar 

  5. Aranjuelo I et al (2011) Does ear C sink strength contribute to overcoming photosynthetic acclimation of wheat plants exposed to elevated CO2? J Exp Bot 62:3957–3969

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  6. Bunce JA (2001) Direct and acclimatory responses of stomatal conductance to elevated carbon dioxide in four herbaceous crop species in the field. Glob Change Biol 7:323–331

    Article  Google Scholar 

  7. Caldiz D, Clua A, Beltrano J, Tenenbaum S (1998) Ground cover, photosynthetic rate and tuber yield of potato (Solanum tuberosum L.) crops from seed tubers with different physiological age modified by foliar applications of plant growth regulators. Potato Res 41:175–185

    Article  Google Scholar 

  8. Cao W, Tibbitts T, Wheeler R (1994) Carbon dioxide interactions with irradiance and temperature in potatoes. Adv Space Res 14:243–250

    CAS  PubMed  Article  Google Scholar 

  9. Chen C-T, Setter TL (2012) Response of potato dry matter assimilation and partitioning to elevated CO2 at various stages of tuber initiation and growth. Environ Exp Bot 80:27–34

    CAS  Article  Google Scholar 

  10. Davey PA, Hunt S, Hymus GJ, DeLucia EH, Drake BG, Karnosky DF, Long SP (2004) Respiratory oxygen uptake is not decreased by an instantaneous elevation of [CO2], but is increased with long-term growth in the field at elevated [CO2]. Plant Physiol 134:520–527

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  11. Davies WJ, Kudoyarova G, Hartung W (2005) Long-distance ABA signaling and its relation to other signaling pathways in the detection of soil drying and the mediation of the plant’s response to drought. J Plant Growth Regul 24:285–295

    CAS  Article  Google Scholar 

  12. Donnelly A, Craigon J, Black CR, Colls JJ, Landon G (2001) Elevated CO2 increases biomass and tuber yield in potato even at high ozone concentrations. New Phytol 149:265–274

    Article  Google Scholar 

  13. Drake BG, Gonzàlez-Meler MA, Long SP (1997) More efficient plants: a consequence of rising atmospheric CO2? Ann Rev Plant Biol 48:609–639

    CAS  Article  Google Scholar 

  14. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    CAS  Article  Google Scholar 

  15. Ewing EE (1995) The role of hormones in potato (Solanum tuberosum L.) tuberization. In: Plant Hormones. Springer, pp 698–724

  16. Ewing E, Struik P (2010) Tuber formation in potato: induction, initiation, and growth. Hort Rev 14:197

    Google Scholar 

  17. Finnan JM, Donnelly A, Burke JI, Jones MB (2002) The effects of elevated concentrations of carbon dioxide and ozone on potato (Solanum tuberosum L.) yield. Agric Ecosyst Environ 88:11–22

    CAS  Article  Google Scholar 

  18. Finnan J, Donnelly A, Jones M, Burke J (2005) The effect of elevated levels of carbon dioxide on potato crops: a review. J Crop Improv 13:91–111

    CAS  Article  Google Scholar 

  19. Fischer L, Lipavska H, Hausman J-F, Opatrny Z (2008) Morphological and molecular characterization of a spontaneously tuberizing potato mutant: an insight into the regulatory mechanisms of tuber induction. BMC Plant Biol 8:117

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  20. Fleisher DH, Barnaby J, Sicher R, Resop JP, Timlin D, Reddy V (2014) Potato gas exchange response to drought cycles under elevated carbon dioxide. Agron J 106:2024–2034

    CAS  Article  Google Scholar 

  21. Foyer CH, Shigeoka S (2011) Understanding oxidative stress and antioxidant functions to enhance photosynthesis. Plant Physiol 155:93–100. https://doi.org/10.1104/pp.110.166181

    CAS  Article  PubMed  Google Scholar 

  22. Fuentes D et al (2011) A deficiency in the flavoprotein of Arabidopsis mitochondrial complex II results in elevated photosynthesis and better growth in nitrogen-limiting conditions. Plant Physiol 157:1114–1127

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  23. Geiger DR, Servaites JC (1991) Carbon allocation and response to stress. In: Mooney H, Winner W, Pell E (eds) Response of plants to multiple stresses. Academic Publishers, San Diego, pp 103–127

    Google Scholar 

  24. Genty B, Briantais J-M, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta Gen Subj 990:87–92

    CAS  Article  Google Scholar 

  25. Gifford RM (2004) The CO2 fertilising effect–does it occur in the real world? New Phytol 163:221–225

    Article  Google Scholar 

  26. Gomez-Casanovas N, Blanc-Betes E, Gonzalez-Meler MA, Azcon-Bieto J (2007) Changes in respiratory mitochondrial machinery and cytochrome and alternative pathway activities in response to energy demand underlie the acclimation of respiration to elevated CO2 in the invasive Opuntia ficus-indica. Plant Physiol 145:49–61

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  27. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. vol 347. Circular. California Agricultural Experiment Station, vol 2nd edit.

  28. Högy P, Fangmeier A (2009) Atmospheric CO2 enrichment affects potatoes: 1. Aboveground biomass production and tuber yield. Europ J Agron 30:78–84

    Article  CAS  Google Scholar 

  29. Kaminski KP, Kørup K, Nielsen KL, Liu F, Topbjerg HB, Kirk HG, Andersen MN (2014) Gas-exchange, water use efficiency and yield responses of elite potato (Solanum tuberosum L.) cultivars to changes in atmospheric carbon dioxide concentration, temperature and relative humidity. Agric Forest Meteorol 187:36–45

    Article  Google Scholar 

  30. Katny MAC, Hoffmann-Thoma G, Schrier AA, Fangmeier A, Jäger H-J, van Bel AJ (2005) Increase of photosynthesis and starch in potato under elevated CO2 is dependent on leaf age. J Plant Physiol 162:429–438

    CAS  PubMed  Article  Google Scholar 

  31. Kloosterman B, Vorst O, Hall RD, Visser RG, Bachem CW (2005) Tuber on a chip: differential gene expression during potato tuber development. Plant Biotechnol J 3:505–519

    CAS  PubMed  Article  Google Scholar 

  32. Knudson LL, Tibbitts TW, Edwards GE (1977) Measurement of ozone injury by determination of leaf chlorophyll concentration. Plant Physiol 60:606–608

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  33. LeNoble ME, Spollen WG, Sharp RE (2004) Maintenance of shoot growth by endogenous ABA: genetic assessment of the involvement of ethylene suppression. J Exp Bot 55:237–245

    CAS  PubMed  Article  Google Scholar 

  34. Li X et al. (2013) Stimulated leaf dark respiration in tomato in an elevated carbon dioxide atmosphere. Sci Rep 3

  35. Long SP, Ainsworth EA, Rogers A, Ort DR (2004) Rising atmospheric carbon dioxide: plants FACE the Future*. Ann Rev Plant Biol 55:591–628

    CAS  Article  Google Scholar 

  36. Palmer C, Smith O (1970) Effect of kinetin on tuber formation on isolated stolons of Solanum tuberosum L. cultured in vitro. Plant Cell Physiol 11:303–314

    CAS  Article  Google Scholar 

  37. Piñero MC, Houdusse F, Garcia-Mina JM, Garnica M, del Amor FM (2014) Regulation of hormonal responses of sweet pepper as affected by salinity and elevated CO2 concentration. Physiol Plant 151:375–389

    PubMed  Article  CAS  Google Scholar 

  38. Pospíšilová J (2003) Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biol Plant 46:491–506

    Article  Google Scholar 

  39. Pospisilova J, Vagner M, Malbeck J, Travnickova A, Batkova P (2005) Interactions between abscisic acid and cytokinins during water stress and subsequent rehydration. Biol Plant 49:533–540

    CAS  Article  Google Scholar 

  40. Ramawat KG, Merillon J-M (2013) Bulbous plants: biotechnology. CRC Press

  41. Reddy AR, Rasineni GK, Raghavendra AS (2010) The impact of global elevated CO2 concentration on photosynthesis and plant productivity. Curr Sci pp 46–57

  42. Reinoso H, Travaglia C, Bottini R (2011) ABA increased soybean yield by enhancing production of carbohydrates and their allocation in seed. INTECH Open Access Publisher

  43. Rodríguez-Falcón M, Bou J, Prat S (2006) Seasonal control of tuberization in potato: conserved elements with the flowering response. Ann Rev Plant Biol 57:151–180

    Article  CAS  Google Scholar 

  44. Romanov G (2009) How do cytokinins affect the cell? Russ J Plant Physiol 56:268–290

    CAS  Article  Google Scholar 

  45. Roosta H, Vazirinasab S, Raghami M (2015) Effect of 6-benzylaminopurine and cycocel on minituber production in two potato cultivars In Vitro. Iran J Hotr Sci 46:141–156

    Google Scholar 

  46. Sansberro PA, Mroginski LA, Bottini R (2004) Foliar sprays with ABA promote growth of Ilex paraguariensis by alleviating diurnal water stress. Plant Growth Regul 42:105–111

    CAS  Article  Google Scholar 

  47. Schlegel H-G (1956) Die verwertung organischer säuren durch Chlorella im licht. Planta 47:510–526

    CAS  Article  Google Scholar 

  48. Sharma AK, Pandey K (2013) Potato mini-tuber production through direct transplanting of in vitro plantlets in green or screen houses—a review. Potato J 40

  49. Struik PC, Wiersema SG (2012) Seed potato technology. Wageningen Academic Publication

  50. Taiz L, Zeiger E (2006) Plant Physiology, 4 eds. Sinauer Associates, Inc., Sunderland, Massachusetts

  51. Thinh NC, Shimono H, Kumagai E, Kawasaki M (2017) Effects of elevated CO2 concentration on growth and photosynthesis of Chinese yam under different temperature regimes. Plant Prod Sci 20:227–236

    CAS  Article  Google Scholar 

  52. Vandermeiren K, Black C, Lawson T, Casanova M, Ojanperä K (2002) Photosynthetic and stomatal responses of potatoes grown under elevated CO2 and/or O3—results from the European CHIP-programme. Europ J Agron 17:337–352

    CAS  Article  Google Scholar 

  53. Wang X, Anderson OR, Griffin KL (2004) Chloroplast numbers, mitochondrion numbers and carbon assimilation physiology of Nicotiana sylvestris as affected by CO2 concentration. Environ Exp Bot 51:21–31

    CAS  Article  Google Scholar 

  54. Wang X et al (2015) Effects of exogenous GA3 on wheat cold tolerance. J Agric Sci Technol 17:921–934

    Google Scholar 

  55. Yong JW, Wong SC, Letham DS, Hocart CH, Farquhar GD (2000) Effects of elevated [CO2] and nitrogen nutrition on cytokinins in the xylem sap and leaves of cotton. Plant Physiol 124:767–780

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  56. Yuhui W, Denghua Y, Junfeng W, Yi D, Xinshan S (2017) Effects of elevated CO2 and drought on plant physiology, soil carbon and soil enzyme activities. Pedosphere 27:846–855

    Article  CAS  Google Scholar 

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Correspondence to Mohammad Javad Ahmadi-Lahijani.

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Ahmadi-Lahijani, M.J., Kafi, M., Nezami, A. et al. ABA and BAP improve the accumulation of carbohydrates and alter carbon allocation in potato plants at elevated CO2. Physiol Mol Biol Plants (2021). https://doi.org/10.1007/s12298-021-00956-w

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Keywords

  • Dark respiration
  • Hydroponics
  • Photosynthetic rate
  • Photosynthetic pigments
  • Soluble carbohydrates
  • Starch