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Physiological responses and expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress

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Abstract

Faba bean (Vicia faba L.) is the major food legume crop in Tunisia. However, its growth and yield is strongly affected by water‐limited environments. In this study, osmotic stress exhibited a negative effect on Bachar and Badii cultivar. Nevertheless, the deteriorating effects of osmotic stress were relatively low on studied parameters of Bachar due to its better efficiency to reduce oxidative damage by increasing enzymatic activities such as catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX), accumulation of total chlorophyll (Chlt), soluble sugars and leaf relative water content (RWC). GC–MS analysis determined a total of 11 soluble carbohydrates induced by osmotic stress and differentially accumulated in the both cultivars. Bachar showed elevated levels of mannose, glucose, galactose, ribose, rhamnose and myo-inositol which might help to maintain osmotic adjustment, membranes and proteins protection from the damaging effect of reactive oxygen species. Sugar metabolism related genes (VfNINV3, VfPHS2, VfFRK4, VfHXK1, VfGPI1, VfSTP1.1, VfpGlcT1.1, VfSTP5.1, VfpGlcT1.2, VfSWEET2.1, VfVINV2, VfSUS1, VfPGM1, VfSUT1.1, VfGPT1, VfSPS1, VfSPP1, VfPHS1, VfSUT4.1 and VfTMT1.1) were differentially expressed in both cultivars demonstrating their important roles in sugar accumulation. Most of these genes were upregulated in the leaves of Bachar under moderate and severe stress, which could lead to increase glycolysis and tricarboxylic acid cycle in order to accelerate energy production, necessary to increase osmotic regulation and consequently enhancing the osmotic stress tolerance in that cultivar. Overall, sugars accumulation ability can be used as a useful indicator for the osmotic stress tolerant potential in faba bean breeding programs.

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Authors and Affiliations

  1. Laboratory of Legumes, Centre of Biotechnology of Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Emna Ghouili, Moez Jebara, Salwa Harzalli Jebara, Souhir Abdelkarim, Oumaima Chaieb, Fatma Souissi & Ghassen Abid

  2. Laboratory of Agronomy, National Agronomy Institute of Tunisia (INAT), University of Carthage, Avenue Charles Nicolle, BP 43, 1082, Tunis-Mahrajène, Tunisia

    Khaled Sassi

  3. Laboratory of Integrated Olive Production, Olive Tree Institute, BP 208, 1082, Tunis-Mahrajène, Tunisia

    Yassine Hidri

  4. Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Rim Nefissi Ouertani

  5. Department of Life Sciences, Walloon Agricultural Research Centre, Chaussée de Charleroi, BP 234, 5030, Gembloux, Belgium

    Yordan Muhovski

  6. Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Mohamed El Ayed & Selim Jallouli

  7. Department of Horticultural Sciences and Vegetable Crops, High Institute of Agronomy of Chott Mariem, University of Sousse, 4042, Sousse, Tunisia

    Fatma Kalleli & Mahmoud M’hamdi

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  1. Emna Ghouili
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Ghouili, E., Sassi, K., Jebara, M. et al. Physiological responses and expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress. Physiol Mol Biol Plants 27, 135–150 (2021). https://doi.org/10.1007/s12298-021-00935-1

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