Evaluation of antioxidant bioindicators and growth responses in Malva parviflora L. exposed to cadmium
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In this study, the effect of cadmium (Cd) uptake and concentration on some growth and biochemical responses were investigated in Malva parviflora under Cd treatments including 0, 10, 50 and 100 µM. The shoots and roots were able to accumulate Cd. However, increased Cd dose led to a considerable Cd content in the roots. Cd stress decreased growth, increased lipid peroxidation and also enhanced proline and ascorbic acid contents in both shoots and roots. Chlorophyll and carotenoid contents decreased in the plants with the increasing Cd concentration. While the activities of catalase (CAT) and superoxide dismutase (SOD) increased in the shoots under different Cd doses, these activities decreased in the roots as compared to the control. Both shoots and roots demonstrated a significant increase in guaiacol peroxidase activity in response to Cd stress. Contrary to the aboveground parts, the roots subjected to Cd doses showed a rise in protein content. Despite higher Cd content in the roots, it seems that CAT and SOD do not play a key role in detoxification of Cd-induced oxidative stress. These findings confirm that reduced biomass and growth under Cd stress can be due to an increase in oxidative stress and a decrease in photosynthetic pigment content. The present study clearly indicates that the shoots and roots exploit different tolerance behaviors to alleviate Cd-induced oxidative stress in M. parviflora.
KeywordsAscorbate Heavy metal Photosynthetic pigments Proline Oxidative stress
Thiobarbituric acid reactive substances
Potassium phosphate buffer
Reactive oxygen species
This work was supported by the Research Affairs of Shahid Chamran University of Ahvaz (Grant No. 94/3/02/31579).
PZ designed and conducted the research, analyzed the data and wrote the paper; RJ performed all the assessments related to growth and biochemical parameters; EN measured cadmium concentration of plant tissues; PH and SR helped as consultant to carry out measurement protocols.
Compliance with ethical standards
Conflict of interest
The authors declared that they have no conflict of interest.
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