Physiology and Molecular Biology of Plants

, Volume 24, Issue 6, pp 1231–1243 | Cite as

Mapping QTLs for physiological and biochemical traits related to grain yield under control and terminal heat stress conditions in bread wheat (Triticum aestivum L.)

  • Faramarz Sohrabi Chah Hassan
  • Mahmood SoloukiEmail author
  • Barat Ali Fakheri
  • Nafiseh Mahdi Nezhad
  • Bahram Masoudi
Research Article


In order to detect genomic regions with different effects for some of the physiological and biochemical traits of wheat, four experiments were conducted at Research Farm of Agricultural and Natural Resources Research Center of Zabol in 2015–2016 and 2016–2017 growing seasons. The experiments were carried out using four alpha lattice designs with two replications under non-stress and terminal heat stress conditions. Plant materials used in this study included 167 recombinant inbred lines and their parents (‘SeriM82’ and ‘Babax’). Six traits including grain yield (GY), proline content (PRO), water soluble carbohydrates (WSC), maximum efficiency of photosystem II (Fv/Fm), cytoplasmic membrane stability (CMS) and chlorophyll content (CHL) were evaluated. Genetic linkage map consisted of 211 AFLP marker, 120 SSR marker and 144 DArT markers with 1864 cm length and 4.4 cm mean distance. QTL analysis was carried out using a mixed-model-based composite interval mapping (MCIM) method. By the combined analysis of normal phenotypic values, 27 additive QTLs and five pairs of epistatic effects were identified for studied traits, among which two additive and one epistatic QTL showed significant QTL × environment interactions. By the combined analysis of stress phenotypic values, a total of 26 QTLs with additive effects and 5 epistatic QTLs were detected, among which one additive and one epistatic QTL showed QTL × environment interactions. Six QTLs with major effects (QGY-2B, QGY-2D, QPro-5B, QWSC-4A, QFv/Fm-6A and QCMS-4B), which were common between two conditions could be useful for marker-assisted selection (MAS) in order to develop heat tolerant and high-performance wheat varieties.


Epistatic QTL Heat stress Recombinant inbred line Grain yield 



The authors are very grateful to Dr. Lynne McIntyre for providing the marker data of SeriM82/Babax population. The Center of Agricultural Biotechnology, University of Zabol, Zabol, Iran is gratefully acknowledged for providing laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

12298_2018_590_MOESM1_ESM.pdf (149 kb)
Supplementary material 1 (PDF 149 kb)


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Faramarz Sohrabi Chah Hassan
    • 1
  • Mahmood Solouki
    • 1
    Email author
  • Barat Ali Fakheri
    • 1
  • Nafiseh Mahdi Nezhad
    • 1
  • Bahram Masoudi
    • 2
  1. 1.Department of Plant Breeding and Biotechnology, Faculty of AgricultureUniversity of ZabolZabolIran
  2. 2.Seed and Plant Improvement InstituteAgricultural Research, Education and Extension Organization (AREEO)KarajIran

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