Physiology and Molecular Biology of Plants

, Volume 24, Issue 6, pp 1147–1164 | Cite as

Characterization of backcross introgression lines derived from Oryza nivara accessions for photosynthesis and yield

  • Yadavalli Venkateswara Rao
  • Divya BalakrishnanEmail author
  • Krishnam Raju Addanki
  • Sukumar Mesapogu
  • Thuraga Vishnu Kiran
  • Desiraju Subrahmanyam
  • Sarla Neelamraju
  • Sitapathi Rao Voleti
Research Article


Improvement of photosynthetic traits is a promising strategy to break the yield potential barrier of major food crops. Leaf photosynthetic traits were evaluated in a set of high yielding Oryza sativa, cv. Swarna × Oryza nivara backcross introgression lines (BILs) along with recurrent parent Swarna, both in wet (Kharif) and dry (Rabi) seasons in normal irrigated field conditions. Net photosynthesis (PN) ranged from 15.37 to 23.25 µmol (CO2) m−2 s−1 in the BILs. Significant difference in PN was observed across the seasons and genotypes. Six BILs showed high photosynthesis compared with recurrent parent in both seasons. Chlorophyll content showed minimum variation across the seasons for any specific BIL but significant variation was observed among BILs. Significant positive association between photosynthetic traits and yield traits was observed, but this association was not consistent across seasons mainly due to contrasting weather parameters in both seasons. BILs 166s and 248s with high and consistent photosynthetic rate exhibited stable high yield levels in both the seasons compared to the recurrent parent Swarna. There is scope to exploit photosynthetic efficiency of wild and weedy rice to identify genes for improvement of photosynthetic rate in cultivars.


BILs Oryza nivara Photosynthesis Seasonal variation Yield 


Chl a

Chlorophyll a content (mg g−1 fresh mass)

Chl b

Chlorophyll b content (mg g−1 fresh mass)

Chl a + b

Total chlorophyll content (mg g−1 fresh mass)


Net photosynthetic rate per unit leaf surface area (µmol CO2 m−2 s−1)


Internal CO2 concentration (µmol CO2 mol−1)


Transpiration rate per unit leaf surface area (mmol H2O m−2 s−1)


Stomatal conductance (mol H2O m−2 s−1)

PN/Ci (CE)

Carboxylation efficiency (µmol CO2 mol−1 air)


Water use efficiency (µmol CO2 mmol−1 H2O)

PN/gs (iWUE)

Intrinsic water use efficiency (µmol CO2 mol−1 H2O)


Specific leaf area (cm2 mg−1)


Specific leaf mass (mg cm−2)


Total dry matter (g plant−1)


Harvest index (%)



This research was conducted in project (ABR/CI/BT/11) on Mapping Quantitative Trait Loci (QTLs) for yield and related traits using backcross inbred lines (BILs) from Elite × Wild crosses of rice (Oryza sativa L.) as part of ICAR- National Professor Project (F.No: Edn/27/4/NP/2012-HRD) funded by Indian Council of Agricultural Research, New Delhi, India. These lines were developed in Department of Biotechnology (DBT) funded project (BT/AB/FG-2 (Ph-II) 2009), New Delhi, India. The authors are highly grateful to the Director, ICAR- IIRR for providing facilities.

Author contributions

DB and SN conceived and planned the work. YVR, AKR, SM and TVK performed phenotypic and genotypic screening. DB and DS analyzed the data. YVR, DB and DS drafted the manuscript. SRV and SN made revisions and approved the final version of the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12298_2018_575_MOESM1_ESM.docx (167 kb)
Supplementary material 1 (DOCX 167 kb)


  1. Ambavaram MMR, Basu S, Krishnan A, Ramegowda V, Batlang U, Rahman L, Baisakh N, Pereira A (2014) Coordinated regulation of photosynthesis in rice increases yield and tolerance to environmental stress. Nat Commun 5:5302CrossRefGoogle Scholar
  2. Ashraf M, Harris PJC (2013) Photosynthesis under stressful environments: an overview. Photosynthetica 51:163–190CrossRefGoogle Scholar
  3. Avenson TJ, Cruz JA, Kanazawa A, Kramer DM (2005) Regulating the proton budget of higher plant photosynthesis. Proc Natl Acad Sci USA 102:9709–9713CrossRefGoogle Scholar
  4. Borjigidai A, Hikosaka K, Hirose T, Hasegawa T, Okada M, Kobayashi K (2006) Seasonal changes in temperature dependence of photosynthetic rate in rice under a free-air CO2 enrichment. Ann Bot 97:549–557CrossRefGoogle Scholar
  5. Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560CrossRefGoogle Scholar
  6. Ding ZJ, Yan JY, Xu XY, Yu DQ, Li GX, Zhang SQ, Zheng SJ (2014) Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis. Plant J 79:13–27CrossRefGoogle Scholar
  7. Divya B, Subrahmanyam D, Badri J, Raju AK, Rao VY, Kavitha B, Sukumar M, Malathi S, Revathi P, Padmavathi G, Babu VR, Sarla N (2016) Genotype × environment interactions of yield traits in backcross introgression lines derived from Oryza sativa cv. Front Plant Sci, Swarna/Oryza nivara. CrossRefGoogle Scholar
  8. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  9. Driever SM, Lawson T, Andralojc PJ, Raines CA, Parry MAJ (2014) Natural variation in photosynthetic capacity, growth, and yield in 64 field-grown wheat genotypes. J Exp Bot 65:4959–4973CrossRefGoogle Scholar
  10. Dwivedi SK, Kumar S, Prakash V, Mondal S, Mishr JS (2015) Influence of rising atmospheric CO2 concentrations and temperature on morpho-physiological traits and yield of rice genotypes in sub humid climate of Eastern India. Am J Plant sci 6:2339–2349CrossRefGoogle Scholar
  11. Evans JR, Jakobsen I, Ogren E (1993) Photosynthetic light response curves. Gradients of light absorption and photosynthetic capacity. Planta 189:191–200CrossRefGoogle Scholar
  12. Fischer RA, Edmeades GO (2010) Breeding and cereal yield progress. Crop Sci 50:S85–S98CrossRefGoogle Scholar
  13. Flexas J, Bota J, Loreto F, Cornic G, Sharkey TD (2004) Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. Plant Biol 6:269–279CrossRefGoogle Scholar
  14. Gaikwad KB, Singh N, Bhatia D, Kaur R, Bains NS, Bharaj TS, Singh K (2014) Yield-enhancing heterotic QTL transferred from wild species to cultivated rice Oryza sativa L. PLoS ONE 9:e96939CrossRefGoogle Scholar
  15. Gibson K, Park JS, Nagaia Y, Hwanga SK, Chod YC, Roh KH, Lee SM, Kim DH, Choie SB, Ito H, Edwards GE, Okita TW (2011) Exploiting leaf starch synthesis as a transient sink to elevate photosynthesis, plant productivity and yields. Plant Sci 181:275–281CrossRefGoogle Scholar
  16. Giuliani R, Koteyeva N, Voznesenskaya E, Evans MA, Cousins AB, Edwards GE (2013) Coordination of leaf photosynthesis, transpiration, and structural traits in rice and wild relatives (Genus Oryza). Plant Physiol 162:1632–1651CrossRefGoogle Scholar
  17. Gu J, Yin X, Stomph TJ, Wang H, Struik PC (2012) Physiological basis of genetic variation in leaf photosynthesis among rice (Oryza sativa L.) introgression lines under drought and well-watered conditions. J Exp Bot 63:5137–5153CrossRefGoogle Scholar
  18. Haritha G, Vishnukiran T, Yugandhar P, Sarla N, Subrahmanyam D (2017) Introgressions from Oryza rufipogon increase photosynthetic efficiency of KMR3 rice lines. Rice Sci 24(2):85–96CrossRefGoogle Scholar
  19. Hassan MS, Khair A, Haqu MM, Azad AK, Hamid A (2009) Genotypic variation in traditional rice varites for chlorophyll content, SPAD value and nitrogen use efficiency. Bangaladesh J Agric Res 34:505–515Google Scholar
  20. He HB, Yang R, Jia B, Chen L, Fan H, Cui J, Yang D, Li ML, Ma FY (2014) Rice photosynthetic productivity and PSII photochemistry under non-flooded irrigation. The Sci World J 1:171–192. CrossRefGoogle Scholar
  21. Hetherington AM, Woodward FI (2003) The role of stomata in sensing and driving environmental change. Nature 424:901–908CrossRefGoogle Scholar
  22. Hirasawa T, Iida Y, Ishihara K (1988) Effect of leaf water potential and air humidity on photosynthetic rate and diffusive conductance in rice plants. Jpn J Crop Sci 57:112–118CrossRefGoogle Scholar
  23. Horton P (2000) Prospects for crop improvement through the genetic manipulation of photosynthesis: morphological and biochemical aspects of light capture. J Exp Bot 51:475–485CrossRefGoogle Scholar
  24. Ishii R (1995) Cultivar differences. In: Matuso T, Kumazawa K, Ishii R, Ishihara K, Hirata H (eds) Science of the rice plant. 2. Physiology. Food and Agriculture Policy Research Center, Tokyo, pp 566–572Google Scholar
  25. Kanemura T, Homma K, Ohsumi A, Shiraiwa T, Horie T (2007) Evaluation of genotypic variation in leaf photosynthetic rate and its associated factors by using rice diversity research set of germplasm. Photosynth Res 94:23–30CrossRefGoogle Scholar
  26. Kawamitsu Y, Agata W (1987) Varietal differences in photosynthetic rate, transpiration rate and leaf conductance for leaves of rice plants. Jpn J Crop Sci 56:563–570CrossRefGoogle Scholar
  27. Kimball BA, Kobayashi K, Bindi M (2002) Responses of agricultural crops to free-air CO2 enrichment. Adv Agron 77:293–368CrossRefGoogle Scholar
  28. Kiran TV, Rao YV, Subrahmanyam D, Rani NS, Bhadana VP, Rao PR, Voleti SR (2013) Variation in leaf photosynthetic characteristics in wild rice species. Photosynthetica 51:350–358CrossRefGoogle Scholar
  29. Kusumi K, Hirotsuka S, Kumamaru T, Iba K (2012) Increased leaf photosynthesis caused by elevated stomatal conductance in a rice mutant deficient in SLAC1, a guard cell anion channel protein. J Exp Bot 63:5635–5644CrossRefGoogle Scholar
  30. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592CrossRefGoogle Scholar
  31. Long SP (2014) We need winners in the race to increase photosynthesis in rice, whether from conventional breeding, biotechnology or both. Plant, Cell Environ 37:19–21CrossRefGoogle Scholar
  32. Long SP, Marshall-Colon A, Zhu XG (2015) Meeting the global food demand of the future by engineering crop photosynthesis and yield potential. Cell 161(1):56–66CrossRefGoogle Scholar
  33. Malathi S, Divya B, Sukumar M, Raju A K, Rao YV, Tripura Venkata VGN, Sarla N (2017) Identification of major effect QTLs for agronomic traits and CSSLs in rice from Swarna/O nivara derived backcross inbred lines. Front Plant Sci 8:1027CrossRefGoogle Scholar
  34. Mann CC (1999) Crop scientists seek a new revolution. Science 283:310–316CrossRefGoogle Scholar
  35. Masumoto C, Ishii T, Kataoka S, Hatanaka T, Uchida N (2004) Enhancement of rice leaf photosynthesis by crossing between cultivated rice, Oryza sativa, and wild rice species, Oryza rufipogon. Plant Prod Sci 7:252–259CrossRefGoogle Scholar
  36. Mitchell PL, Sheehy JE (2006) Supercharging rice photosynthesis to increase yield. New Phytol 171:688–693CrossRefGoogle Scholar
  37. Niinemets Ü, Diaz-Espejo A, Flexas J, Galmés J, Warren CR (2009) Role of mesophyll diffusion conductance in constraining potential photosynthetic productivity in the field. J Exp Bot 60:2249–2270CrossRefGoogle Scholar
  38. Nowak RS, Ellsworth DS, Smith SD (2004) Functional responses of plants to elevated atmospheric CO2: do photosynthetic and productivity data from FACE experiments support early predictions? New Phytol 162:253–280CrossRefGoogle Scholar
  39. Ohno Y (1976) Varietal differences of photosynthetic efficiency and dry matter production in indica rice. Tech Bull TARC 9:1–72Google Scholar
  40. Ohsumi A, Hamasaki A, Nakagawa H, Yoshida H, Shiraiwa T, Horie T (2007) A model explaining genotypic and ontogenetic variation of leaf photosynthetic rate in rice (Oryza sativa) based on leaf nitrogen content and stomatal conductance. Ann Bot-London 99:265–273CrossRefGoogle Scholar
  41. Pawar GS, Padma V, Subrahmanyam D, Kumar SS, Bhave MHV (2015) Association of various morphophenological traits with yield among various rice (Oryza sativa L.) genotypes. J of Res PJTSAU 43:5–10Google Scholar
  42. Radin JW, Hartung W, Kimball BA, Mauney JR (1988) Correlation of stomatal conductance with photosynthetic capacity of cotton only in a CO2-enriched atmosphere: mediation by abscisic acid? Plant Physiol 88(4):1058–1062CrossRefGoogle Scholar
  43. Reynolds MP, Pellegrineschi A, Skovmand B (2005) Sink-limitation to yield and biomass: a summary of some investigations in spring wheat. Ann Appl Biol 146:39–49CrossRefGoogle Scholar
  44. Richards RA (2000) Selectable traits to increase crop photosynthesis and yield of grain crops. J Exp Bot 51:447–458CrossRefGoogle Scholar
  45. Sailaja B, Subrahmanyam D, Neelamraju S, Vishnukiran T, Rao YV, Vijayalakshmi P, Voleti SR, Bhadana VP, Mangrauthia SK (2015). Integrated physiological, biochemical, and molecular analysis identifies important traits and mechanisms associated with differential response of rice genotypes to elevated temperature. Front Plant Sci 6:1044CrossRefGoogle Scholar
  46. Sasaki H, Ishii R (1992) Cultivar differences in leaf photosynthesis of rice bred in Japan. Photosynth Res 32:139–146CrossRefGoogle Scholar
  47. Scafaro AP, Von Caemmerer S, Evans JR, Atwell BJ (2011) Temperature response of mesophyll conductance in cultivated and wild Oryza species with contrasting mesophyll cell wall thickness. Plant, Cell Environ 34(11):1999–2008CrossRefGoogle Scholar
  48. Sharkey TD, Raschke K (1981) Separation and measurement of direct and indirect effects of light on stomata. Plant Physiol 68:33–40CrossRefGoogle Scholar
  49. Shearman VJ, Sylvester-Bradley R, Scott PK, Foulkes MJ (2005) Physiological processes associated with wheat yield progress in the UK. Crop Sci 45:175–185Google Scholar
  50. Steinbauer MJ (2001) Specific leaf weight as an indicator of juvenile leaf toughness in Tasmanian bluegum (Eucalyptus globulus ssp. globulus): implications for insect defoliation. Aust For 64:32–37CrossRefGoogle Scholar
  51. Subrahmanyam D (2002) Interrelationship between leaf gas-exchange characteristics, area leaf mass, and yield in soybean (Glycine max L. Merr) genotypes. Photosynthetica 40(3):445–447CrossRefGoogle Scholar
  52. Swamy BPM, Sarla N (2008) Yield enhancing QTLs from wild species. Biotechnol Adv 26:106–120CrossRefGoogle Scholar
  53. Swamy BPM, Kaladhar K, Ramesha MS, Viraktamath BC, Sarla N (2011) Molecular mapping of QTLs for yield and related traits in Oryza sativa cv Swarna × O. nivara (IRGC81848) backcross population. Rice Sci 18:178–186CrossRefGoogle Scholar
  54. R Core Team (2012) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0.
  55. Teng S, Qian Q, Zeng D, Kunihiro Y, Fujimoto K, Huang D, Zhu L (2004) QTL analysis of leaf photosynthetic rate and related physiological traits in rice (Oryza sativa L.). Euphytica 135:1–7CrossRefGoogle Scholar
  56. Warren CR (2004) The photosynthetic limitation posed by internal conductance to CO2 movement is increased by nutrient supply. J Exp Bot 55:2313–2321CrossRefGoogle Scholar
  57. Wong SC, Cowan IR, Farquhar GD (1985) Leaf conductance in relation to rate of CO2 assimilation. I. Influence of nitrogen nutrition, phosphorus nutrition, photon flux density, and ambient partial pressure of CO2 during ontogeny. Plant Physiol 78:821–825CrossRefGoogle Scholar
  58. Xu DQ, Shen YG (1994) Progress on physiology of crop high production and high efficiency. Science Publishing Company, Beijing, pp 17–23Google Scholar
  59. Yeo ME, Yeo AR, Flowers TJ (1994) Photosynthesis and photorespiration in the genus Oryza. J Exp Bot 45:553–560CrossRefGoogle Scholar
  60. Zhao XQ, Xu J-L, Zhao M, Lafitte R, Zhu L-H, Fu B-Y, Gao YM, Li Z-K (2008) QTLs affecting morph-physiological traits related to drought tolerance detected in overlapping introgression lines of rice (Oryza sativa L.). Plant Sci 174:618–625CrossRefGoogle Scholar
  61. Zhao M, Ding Z, Lafitte R, Sacks E, Dimaygua G, Holt D (2010) Photosynthetic characters in Oryza species. Photosynthetica 48(2):234–240CrossRefGoogle Scholar
  62. Zhu XG, Long SP, Ort DR (2010) Improving photosynthetic efficiency for greater yield. Ann Rev Plant Biol 61:235–261CrossRefGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Yadavalli Venkateswara Rao
    • 1
  • Divya Balakrishnan
    • 1
    Email author
  • Krishnam Raju Addanki
    • 1
  • Sukumar Mesapogu
    • 1
  • Thuraga Vishnu Kiran
    • 2
  • Desiraju Subrahmanyam
    • 2
  • Sarla Neelamraju
    • 1
  • Sitapathi Rao Voleti
    • 2
  1. 1.ICAR- National Professor Project, ICAR- Indian Institute of Rice ResearchHyderabadIndia
  2. 2.Plant Physiology Section, Department of Crop PhysiologyICAR- Indian Institute of Rice ResearchHyderabadIndia

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