Physiology and Molecular Biology of Plants

, Volume 24, Issue 3, pp 493–501 | Cite as

Assessment of genetic diversity and population structure in gladiolus (Gladiolus hybridus Hort.) by ISSR markers

  • Veena Chaudhary
  • Mukesh Kumar
  • Shailendra Sharma
  • Navneet Kumar
  • Vipin Kumar
  • Hemant Kumar Yadav
  • Shiveta Sharma
  • Ujjwal Sirohi
Research Article


ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus (Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80. The dendrogram based UPGMA clustering showed that all the 53 genotypes grouped into three main clusters. Nei’s gene diversity (Na) varied from 0.929 to 1.717, effective number of alleles (Ne) varied from 1.262 to 1.369, Shannon’s information index (I) ranged from 0.251 to 0.359 and gene diversity (He) was in the range from 0.167 to 0.229. Population structure analysis revealed three groups in which 32 genotypes were admixture types.


Gladiolus Molecular diversity Inter simple sequence repeats Population structure 



The authors are grateful to Prf. Gaya Prasad, Hon’ble Vice Chancellor of the Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, U.P. India for providing facilities and encouragement. Also, IIHR, Bangalore, PAU, Ludhiana, NBRI, Lucknow and IARI, New Delhi deserves thanks for providing gladiolus germplasm.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Veena Chaudhary
    • 1
  • Mukesh Kumar
    • 2
  • Shailendra Sharma
    • 3
  • Navneet Kumar
    • 2
  • Vipin Kumar
    • 2
  • Hemant Kumar Yadav
    • 4
  • Shiveta Sharma
    • 3
  • Ujjwal Sirohi
    • 2
  1. 1.CSSS (PG) CollegeMachhra, MeerutIndia
  2. 2.Sardar Vallabhbhai Patel University of Agriculture and TechnologyMeerutIndia
  3. 3.Chaudhary Charan Singh UniversityMeerutIndia
  4. 4.NBRILucknowIndia

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