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Different effects of calcium and penconazole on primary and secondary metabolites of Brassica napus under drought

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Abstract

The effects of penconazole (PEN) and calcium (Ca2+) on physiological and biochemical parameters were investigated in two canola cultivars (RGS003 and Sarigol) under water stress. Drought increased protein content in RGS003, but PEN, Ca2+ and PEN–Ca2+ treatment induced protein content in Sarigol. PEN, Ca2+ and PEN–Ca2+ treatment enhanced soluble sugar content in RGS003. In contrast to Sarigol, drought and PEN treatment induced total phenol content in RGS003. Flavonoid content increased by drought, but Ca2+ and PEN–Ca2+ treatment decreased it in both cultivars. Ca2+ and PEN–Ca2+ treatment enhanced tocopherol content in both cultivars under drought stress. Drought stress increased Phenylalanine ammonia-lyase (PAL) activity in Sarigol. PEN–Ca2+ treatment increased relative expression of PAL and its activity in RGS003. Fatty acid composition was modified by drought, PEN and Ca2+. Saturated fatty acid (stearic acid) content declined but unsaturated fatty acid (oleic acid) content enhanced in both cultivars under drought. The application of PEN and Ca2+ decreased unsaturated fatty acids (linoleic and linolenic acid) in RGS003 under drought. According to our results, PEN and Ca2+ changed physiological and biochemical parameters and therefore these compounds are suggested for reduction of the negative effects of drought stress in canola.

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Acknowledgements

The financial support of this research was provided by College of Science, University of Tehran. We thank Dr. Mehrdad Behmanesh and Dr. Najmeh Ahmadian Chashmi.

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Authors and Affiliations

  1. Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms in Iran, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran

    Maryam Rezayian, Vahid Niknam & Hassan Ebrahimzadeh

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  1. Maryam Rezayian
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  2. Vahid Niknam
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  3. Hassan Ebrahimzadeh
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Maryam Rezayian has contributed in the major bench experiments. Dr. Vahid Niknam and Dr. Hassan Ebrahimzadeh equally designed the experiments. All authors read and approved the manuscript.

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Correspondence to Vahid Niknam.

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Rezayian, M., Niknam, V. & Ebrahimzadeh, H. Different effects of calcium and penconazole on primary and secondary metabolites of Brassica napus under drought. Physiol Mol Biol Plants 25, 497–509 (2019). https://doi.org/10.1007/s12298-018-00634-4

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  • DOI: https://doi.org/10.1007/s12298-018-00634-4

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