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Delayed expression of SAGs correlates with longevity in CMS wheat plants compared to its fertile plants

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Abstract

Reproductive sinks regulate monocarpic senescence in crop plants. Monocarpic senescence was studied in wheat fertile (cv. HW 2041) and its isonuclear cytoplasmic male sterile (CMS) line. CMS plants exhibited slower rate of senescence accompanied by longer green leaf area duration and slower deceleration in chlorophyll, protein content, PN and rubisco content coupled with lower protease activities than fertile (F) plants. CMS plants also exhibited lower ROS levels and less membrane damage than F plants. CMS plants maintained better antioxidant defense, less oxidative damage in chloroplast and higher transcript levels of both rbcL and rbcS genes during senescence than F plants. F plants exhibited early induction and higher expression of SAGs like serine and cysteine proteases, glutamine synthetases GS1 and GS2, WRKY53 transcription factor and decline in transcript levels of CAT1 and CAT2 genes than CMS plants. Hence, using genetically fertile and its CMS line of wheat it is confirmed that delayed senescence in the absence of reproductive sinks is linked with slower protein oxidation, rubisco degradation and delayed activation of SAGs. Better antioxidant defense in chloroplasts at later stages of senescence was able to mitigate the deleterious effects of ROS in CMS plants. We propose that delayed increase in ROS in cytoplasmic male sterile wheat plants resulted in delayed activation of WRKY53, SAGs and the associated biochemical changes than fertile plants.

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Abbreviations

AsA:

Ascorbate

APX:

Ascorbate peroxidase

CP:

Cysteine protease

CAT:

Catalase

DAA:

Days after anthesis

GS:

Glutamine synthetase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

SAGs:

Senescence associated genes

SP:

Serine protease

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Acknowledgments

This research was supported by the financial grants of CSIR Emeritus Scientist Scheme awarded to Dr. (Mrs.) R. K. Chopra.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Stress Physiology Lab, Water Technology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India

    Vimal Kumar Semwal & Renu Khanna-Chopra

  2. Centre for Environment Science and Climate Resilient Agriculture, Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110012, India

    Bhupinder Singh

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  1. Vimal Kumar Semwal
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  2. Bhupinder Singh
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  3. Renu Khanna-Chopra
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Correspondence to Renu Khanna-Chopra.

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Semwal, V.K., Singh, B. & Khanna-Chopra, R. Delayed expression of SAGs correlates with longevity in CMS wheat plants compared to its fertile plants. Physiol Mol Biol Plants 20, 191–199 (2014). https://doi.org/10.1007/s12298-013-0215-1

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