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Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

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Abstract

An optimized protocol was developed for in vitro plant regeneration of a medicinally important herb Hedychium coronarium J. Koenig using sprouted buds of rhizomes. The rhizomes with sprouted bud were inoculated on Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) supplemented with either N6-benzyladenine (BA) alone (1.0–4.0 mg L−1) or in combination with 0.5 mg L−1 naphthalene acetic acid (NAA). Of these combinations, MS supplemented with a combination of 2.0 mg L−1 BA and 0.5 mg L−1 NAA was most effective. In this medium, best shoots (3.6) and roots (4.0) regeneration was observed simultaneously with an average shoot and root length of 4.7 cm and 4.2 cm respectively. Regeneration of shoots and roots in the same medium at the same time (One step shoot and root regeneration) reduced the time for production of in vitro plantlets and eliminates the media cost of rooting. Cent-percent (100 %) success in plant establishment was observed in both gradual acclimatization process as well as when plants were directly transferred to outdoor in clay pots containing a mixture of garden soil and sand (2:1) without any sequential acclimatization stage.

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Fig. 1

Abbreviations

BA:

N6-Benzyladenine

MS:

Murashige and Skoog’s (1962) medium

NAA:

Naphthaleneacetic acid

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Acknowledgment

We thank the anonymous reviewers for their valuable comments to improve the manuscript. Our thanks are also to Dr. Subhra Prakash Das, Reader in English, Ravenshaw University, Cuttack, India for going through the manuscript for improvement of the English. Fund support under FIST program by Department of Science and Technology (DST), Government of India to Department of Botany, Ravenshaw University, Cuttack-753 003, India is gratefully acknowledged (SR/FST/LSI-017-2010).

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  1. Department of Botany, Ravenshaw University, Cuttack, 753003, Odisha, India

    Pritam Mohanty, Shashikanta Behera, Swasti S. Swain, Durga P. Barik & Soumendra K. Naik

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  1. Pritam Mohanty
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  2. Shashikanta Behera
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  3. Swasti S. Swain
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  4. Durga P. Barik
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  5. Soumendra K. Naik
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Correspondence to Soumendra K. Naik.

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Mohanty, P., Behera, S., Swain, S.S. et al. Micropropagation of Hedychium coronarium J. Koenig through rhizome bud. Physiol Mol Biol Plants 19, 605–610 (2013). https://doi.org/10.1007/s12298-013-0199-x

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