Abstract
Improving drought tolerance through gene manipulation has been of importance for modern agriculture, which requires identification and validation of candidate genes. Prospecting candidate genes from drought adapted crop species is of immense significance. To identify candidate stress responsive genes from adapted crop, we carried out expression analysis of a few drought responsive ESTs from Arachis hypogaea L. (peanut). The expression patterns of nine AhDR (Arachis hypogea drought responsive) clones were analysed under drought. Quantitative reverse transcription PCR analysis revealed stress responsive nature of the selected genes. The clones AhDR 118 (putative cyclin T-like), AhDR185 (aldehyde reductase-like), AhDR193 (cholin kinase-like) and AhDR 76 (proline amino peptidase-like) showed more than five fold increase in expression. Highly upregulated genes analysed for expression pattern against salinity at seedling level indicated that these genes provide cross protection. This paper is the first report indicating the association of peanut genes cyclin T, proline amino peptidase and choline kinase to drought tolerance, and the possible roles of these genes are discussed.
This is a preview of subscription content, log in via an institution to check access.
References
Abdeen A, Schnell, Miki B (2010) Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants over expressing the transcription factor. BMC Genomics 11:69
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci 15:413–428
Datta K, Schimidt A, Marcus (1989) Characterization of two soybean repetitive proline rich proteins and a cognate cDNA from germinated axes. Plant Cell 1:945–952
Fulop K, Alada R, Pettko-Szandtner, Magyar Z, Miskolczi P, Kondorosi E, Dudits D, Bako L (2005) The Medicago CDKC;1-CYCLINT;1 kinase complex phosphorylates the carboxy-terminal domain of RNA polymerase II and promotes transcription. Plant J 42:810–820
Gopalakrishna R, Ganeshkumar, Krishnaprasad BT, Mathew MK, Udayakumar M (2001) A stress-responsive gene from peanut, Gdi15, is homologous to flavonol 3-O-glucosyl transferase involved in anthocyanin biosynthesis. Biochem Biophys Res Commun 284:574–579
Govind G, Harshavardhan VT, Patricia JK, Dhanalakshmi R, Senthil JM, Sreenivasulu N, Udaya Kumar M (2009) Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol Genet Genomics 281(6):591–605
Guo B, Chen X, Dang P, Scully BT, Liang X, Holbrook CC, Yu J, Culbreath AK (2008) Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticus infection. BMC Dev Biol 8:12
Hegedus A, Erdei S, Janda T, Toth E, Horvath G, Dudits D (2004) Transgenic tobacco plants overproducing alfalfa aldose/aldehyde reductase show higher tolerance to low temperature and cadmium stress. Plant Sci 166(5):1329–1333
Hideg NT, Oberschall A, Dudits D, Vass I (2003) Detoxification function of aldose/aldehyde reductase during drought and ultraviolet-B (280–320 nm) stresses. Plant Cell Environ 26:513–522
Karaba A, Dixit S, Greco R, Aharoni A, Trijatmiko KR, Marsch-Martinez N, Krishnan A, Nataraja KN, Udayakumar M, Pereira A (2007) Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene. Proc Natl Acad Sci 104:15270–15275
Krasensky J, Jonak C (2012) Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. J Exp Bot 63(4):1523–1524
Nautiyal PC, Rao N, Joshi YC (2002) Moisture-deficit-induced changes in leaf-water content, leaf carbon exchange rate and biomass production in groundnut cultivars differing in specific leaf area. Field Crops Res 74:67–79
Nethra P, Nataraja KN, Rama, Udayakumar M (2006) Standardizations of environmental condition for induction and retention of posttranscriptional gene silencing using tobacco rattle virus vector. Curr Sci 90(3):431–435
Oberschall A, Deaak M, Toeroek K, Sass L, Vass I, Kovaacs I, Fehear A, Dudits D, Horvaath GV (2000) A novel aldose/aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses. Plant J 24(4):437–446
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, volume 1. Cold spring Harbour laboratory press, New York
Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T et al (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292
Szawłowska U, Grabowska A, Zastocka EZ, Bielawski W (2012) TsPAP1 encodes a novel plant prolyl aminopeptidase whose expression is induced in response to suboptimal growth conditions. Biochem Biophys Res Commun 419(1):104–109
Acknowledgments
The authors would like to thank Dr. M. Udayakumar for useful suggestions. The research work was funded by the Department of Biotechnology (DBT) and Indian Council of Agricultural Research (ICAR), Government of India, New Delhi.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pruthvi, V., Rama, N., Govind, G. et al. Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea , L.). Physiol Mol Biol Plants 19, 277–281 (2013). https://doi.org/10.1007/s12298-012-0156-0
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12298-012-0156-0