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Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants

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Abstract

Lawsonia inermis Linn. (Mehandi) is cultivated as cash crop in India particularly in Sojat area of Pali district, Rajasthan. Present investigation describes an efficient regeneration system for elite genotype of L. inermis using nodal segments. Optimum response in terms of percent cultures responding, days to bud break and average shoot length was observed on MS medium supplemented with 6-benzylaminopurine (BA; 2.0 mg l−1). Shoot multiplication was influenced by plant growth regulators, repeated transfer of explants and addition of ammonium sulphate. Maximum shoots were regenerated on MS medium supplemented with BA (0.25 mg l−1), kinetin (Kn; 0.25 mg l−1), indole-3-acetic acid (IAA; 0.1 mg l−1) and ammonium sulphate (150 mg l−1). To reduce resources, time and labours costs, we have also attempted ex vitro rooting of shoots. About 95 % shoots were rooted ex vitro on soilrite after treatment with indole-3-butyric acid (IBA; 300 mg l−1) and 2-naphthoxy acetic acid (NOA; 100 mg l−1) and establishment in soil successfully.

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Abbreviations

Ac:

Activated charcoal

BA:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kn:

Kinetin

NOA:

2-Naphthoxy acetic acid

PPFD:

Photosynthetic photon flux density

RH:

Relative Humidity

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Acknowledgements

Financial assistance provided to the Department of Botany by University Grants Commissions (UGC) of India and the Department of Science and Technology (DST), Govt. of India under SAP (Special Assistance Program) and FIST (Infrastructure Development in Science and Technology) for is greatfully acknowledged. The basic laboratory and greenhouse infrastructure used for research work have been established as Regional Micro- propagation Unit for Arid regions with major funds of Department of Biotechnology (DBT), Govt. of India under networking program on micropropagation. Author (Kheta Ram) is also thankful to Council of Scientific and Industrial Research (CSIR) for awarding fellowship to promotion of research.

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  1. Biotechnology Unit, Department of Botany, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India

    Kheta Ram & N. S. Shekhawat

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  1. Kheta Ram
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  2. N. S. Shekhawat
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Correspondence to Kheta Ram.

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Ram, K., Shekhawat, N.S. Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants. Physiol Mol Biol Plants 17, 281–289 (2011). https://doi.org/10.1007/s12298-011-0069-3

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