Evidence of Association of CYP1A1 Expression in Blood Lymphocytes and Clinicopathological Variables in Oral Cancer

Abstract

Association of smokeless tobacco (SLT) with oral cancer is well documented. The nitrosamines of SLT are metabolically bioactivated by CYP1A1. Therefore, in this pilot study, we investigated association of CYP1A1 expression with polymorphism, clinicopathological variables and outcome in exclusive SLT chewers. Semiquantitative RT-PCR analysis on RNA isolated from peripheral blood lymphocytes of 65 histopathologically confirmed cases revealed increased CYP1A1 mRNA expression in Msp I (CYP1A1*2A) and Ile/Val (CYP1A1*2C) polymorphic variants. A higher trend of CYP1A1 mRNA levels in invasive and recurrent tumors which also associated with shorter disease free and overall survival was seen. Odds ratio analysis indicated the association of higher CYP1A1 mRNA levels with disease aggressiveness i.e. metastasis and invasion. Study revealed wider oncogenic role of CYP1A1 in oral cancer patients and justifies further studies and analysis in a large cohort.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors

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RDS, PSP SJP: conceived and co-designed the study. It was supervised for all the experimental design by JBP; RDS, KAP: carried out the experiments and analyzed the results. Manuscript was drafted by RDS. All authors have read, modified and approved the final version of the manuscript. These authors equally contributed to this work.

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Correspondence to P. S. Patel.

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Singh, R.D., Patel, K.A., Patel, J.B. et al. Evidence of Association of CYP1A1 Expression in Blood Lymphocytes and Clinicopathological Variables in Oral Cancer. Ind J Clin Biochem (2021). https://doi.org/10.1007/s12291-021-00958-1

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Keywords

  • CYP1A1 expression
  • Oral cancer
  • Smokeless tobacco
  • mRNA
  • Polymorphism