Assessment of Garcinia pedunculata Roxb. Role on Biological Markers in Carbon Tetra Chloride Induced Rat Model

Abstract

Garcinia pedunculata Roxb. is an important medicinal plant of North Eastern (NE) region of India, having number of medicinal properties and used against various diseases in folk medicine. An empirical research was designed to carry out evaluation of hepatoprotective activity of the leaves of G. pedunculata with special reference to its putative protective role. Methanolic extract from the dried leaf powder of G. pedunculata Roxb. was prepared by hot continuous extraction method. The prepared extract was investigated at different dose levels for its hepatoprotective nature and further histopathological study was carried out to ascertain the degree of reversing the hepatotoxic manifestation induced by CCl4 (Carbon tetrachloride). LD50 values of the G. pedunculata Roxb. extract was found to be safe up to 2000 mg. The in vivo biological studies on serum and tissues of male Wister rats at the doses of 100 mg, 300 mg and 600 mg/kg body weight respectively was carried out taking Silymarin as standard. The methanolic extract of G. pedunculata Roxb, improved the cholesterol level along with significant improvement of SGPT (Serum Glutamate Pyruvate Transaminase), SGOT (Serum Glutamate Oxaloacetate Transaminase), ALP (Alkaline Phosphatase) and total protein in respect to Silymarin group. The test extract at the dose 600 mg/kg body weight was found to significantly reverse the elevated marker enzymes i.e. SGOT, SGPT, ALP indicating its hepatoprotective role. The higher dose extracts were also found to have pronounced effect on oxidative stress parameters such as GSH and catalase on CCl4 induced rats. The histopathological studies further augmented the protective activity of G. pedunculata leaf extract thereby endorsing the traditional usage of the plant.

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Acknowledgements

The authors acknowledge the infrastructure facilities provided by the Department of Pharmaceutical Sciences, Dibrugarh University.

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Correspondence to Biman Bhuyan.

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Ethical Approval

All procedures performed in studies involving animals were in accordance with the ethical standards promulgated by the Institutional Animal Care Committee, CPCSEA, India. The institutional registration number is 1576/GO/a/11/CPCSE dated: 17/2/2012. The present study is approved vide Approval No. IACE/DU/99 Dated 27/3/2015.

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Bhuyan, B., Deka, T. & Rajak, P. Assessment of Garcinia pedunculata Roxb. Role on Biological Markers in Carbon Tetra Chloride Induced Rat Model. Ind J Clin Biochem 35, 331–338 (2020). https://doi.org/10.1007/s12291-019-00825-0

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Keywords

  • Garcinia pedunculata Roxb.
  • Hepatoprotective
  • Methanolic extract
  • CCl4
  • Histopathology