Indian Journal of Clinical Biochemistry

, Volume 33, Issue 2, pp 171–177 | Cite as

Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats

  • Thangarasu Rajakumar
  • Pachaiappan Pugalendhi
  • Subbaiyan Thilagavathi
Original Article


The present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague–Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased levels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was further confirmed by Periodic Acid Schiff staining in mammary and liver tissues. Upon oral administration of AITC to DMBA injected rats, the abnormal changes were reverted back to near normal levels and biochemical findings are supported by histological analysis. This could be due to the anti-neoplastic potential of AITC against DMBA-induced mammary carcinogenesis. The result shows that AITC has the potential to inhibit abnormal glycosylation that favors neoplastic transformation.


Allyl isothiocyanate 7,12-dimethylbenz(a)anthracene Glycoprotein components Mammary carcinogenesis 



We gratefully acknowledged the financial assistance from Indian Council of Medical Research (ICMR), in the form of Senior Research Fellowship (SRF), New Delhi, India to the first author Mr. T. Rajakumar.


This study was funded by Indian Council of Medical Research (ICMR), Government of India (Grant No. 3/2/2/189/2013/NCD-III (OPA-27129)).

Compliance with Ethical Standards

Conflict of interest

The first author (Mr. T. Rajakumar) has received Senior Research Fellowship (SRF), from Indian Council of Medical Research (ICMR), Government of India. The corresponding author (Dr. P. Pugalendhi) and last author (Mrs. S. Thilagavathi) declares that there are no conflicts of interest.

Ethical Approval

This study was approved by the Institutional Animal Ethics Committee (IAEC), regulated by the Committee for the Purpose of Control and Supervision of Experimental Animals (CPCSEA) (Reg. No. 160/1999/CPCSEA and Proposal No. 983).

Informed Consent

This article does not contain any studies with human participants.


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Copyright information

© Association of Clinical Biochemists of India 2017

Authors and Affiliations

  • Thangarasu Rajakumar
    • 1
  • Pachaiappan Pugalendhi
    • 1
  • Subbaiyan Thilagavathi
    • 1
  1. 1.Department of Biochemistry and Biotechnology, Faculty of ScienceAnnamalai UniversityChidambaramIndia

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