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CRISP Points on Establishing CRISPR-Cas9 In Vitro Culture Experiments in a Resource Constraint Haematology Oncology Research Lab

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Gene editing research has seen rapid growth over the past decade or so, however with the discovery of CRISPR-Cas9 gene editing tool in recent years, the same has witnessed a global interest with many scientists and research groups worldwide carrying out cutting edge experiments to target various diseases and cancers and develop a cure. This has been made possible partially due to the ease of use and flexibility of the CRISPR-Cas9 system as compared to other conventional gene editing tools. Hence, CRISPR-Cas9 has found its way into most basic molecular laboratories and within reach of most low-middle income research groups. Despite these favourable advantages, there exists a cost barrier and lack of proper knowledge and awareness on the correct work flow desired, especially in molecular laboratories looking forward to develop and experiment with high end research. This mini review attempts to iron out these factors and project an algorithmic approach to tide over and establish a workable in vitro gene editing experiment in a resource constraint haematology oncology laboratory setting. However, the basic principle and steps outlined in this review can also be translated for research in any other medical specialty laboratory setting.

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Authors and Affiliations

  1. Pediatric Allergy Immunology Unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India

    Jhumki Das

  2. Pediatric Hematology-Oncology Unit, Advanced Paediatric Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India

    Prateek Bhatia

  3. Pediatric Hematology-Oncology Unit, Department of Pediatrics, Post Graduate Institute of Medical Education and Research, Chandigarh, India

    Aditya Singh

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  1. Jhumki Das
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  2. Prateek Bhatia
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  3. Aditya Singh
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Correspondence to Prateek Bhatia.

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Das, J., Bhatia, P. & Singh, A. CRISP Points on Establishing CRISPR-Cas9 In Vitro Culture Experiments in a Resource Constraint Haematology Oncology Research Lab. Indian J Hematol Blood Transfus 35, 208–214 (2019). https://doi.org/10.1007/s12288-018-1008-z

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  • DOI: https://doi.org/10.1007/s12288-018-1008-z

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