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Study on the Role of Calreticulin Within Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

To observe the differences in proteins between adult patients with chronic immune thrombocytopenic purpura (ITP) and healthy adults. 30 patients with chronic ITP and 30 healthy controls were enrolled into the study. The platelet total protein was extracted from peripheral venous blood of 10 chronic ITP patients and 10 healthy controls respectively, and subjected to two-dimensional electrophoresis (2-DE) to find the differential protein spot between chronic ITP patients and healthy controls, then the differential protein spots were identified by mass spectrometry. Subsequently, platelets RNA and proteins were isolated from the other 20 chronic ITP patients and 20 healthy controls respectively, and used for confirming the 2-DE and mass spectrometry results by using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme linked immunosorbent assay (ELISA). 2-DE combined with mass spectrometry revealed that calreticulin (CRT) expressed normally within platelets from healthy controls, while it reduced within platelets from patients with chronic ITP. qPCR and ELISA confirmed that CRT was decreased at both RNA transcription and protein expression levels within platelets from chronic ITP patients compared with healthy controls. Decreased transcription and expression of CRT within platelets may play an important role in the pathogenesis of chronic ITP, which is worthy of further study.

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References

  1. Palaniappan G, Jennings W (2009) Idiopathic thrombocy-topenic purpura. Mo Med 106(1):69–73

    PubMed  Google Scholar 

  2. Wong CS, Wong VW, Chan CM, Ma BB, Hui EP, Wong MC, Lam MY, Au TC, Chan WH, Cheuk W, Chan AT (2008) Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Oncol Rep 20(1):89–98

    CAS  PubMed  Google Scholar 

  3. Uljon SN, Treon SP, Tripsas CK, Lindeman NI (2013) Challenges with serum protein electrophoresis in assessing progression and clinical response in patients with Waldenström macroglobulinemia. Clin Lymphoma Myeloma Leuk 13(2):247–249

    Article  CAS  Google Scholar 

  4. Chevallet M, Luche S, Rabilloud T (2006) Silver staining of proteins in polyacrylamide gels. Nat Protoc 1(4):1852–1858

    Article  CAS  Google Scholar 

  5. Li HH, Su JH, Chiu CC, Lin JJ, Yang ZY, Hwang WI, Chen YK, Lo YH, Wu YJ (2013) Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade. Mar Drugs 11(7):2625–2642

    Article  Google Scholar 

  6. Sudchada P, Oo-puthinan S, Kerdpin O, Saelim N (2010) ABCB1 gene expression in peripheral blood mononuclear cells in healthy Thai males and females. Genet Mol Res 9(2):1177–1185

    Article  CAS  Google Scholar 

  7. Coppolino MG, Dedhar S (1998) Calreticulin. Int J Biochem Cell Biol 30(5):553–558

    Article  CAS  Google Scholar 

  8. Raghavan M, Wijeyesakere SJ, Peters LR, Del Cid N (2013) Calreticulin in the immune system: ins and outs. Trends Immunol 34(1):13–21

    Article  CAS  Google Scholar 

  9. Baksh S, Michalak M (1991) Expression of calreticulin in Escherichia coli and identification of its Ca2+ binding domains. J Biol Chem 266:21458–21465

    CAS  PubMed  Google Scholar 

  10. McCauliffe DP, Zappi E, Lieu TS, Michalak M, Sontheimer RD, Capra JD (1990) A human Ro/SS-A autoantigen is the homologue of calreticulin and is highly homologous with onchocercal RAL-1 antigen and an aplysia “memory molecule”. J Clin Invest 86(1):332–335

    Article  CAS  Google Scholar 

  11. Gardai SJ, McPhillips KA, Frasch SC, Janssen WJ, Starefeldt A, Murphy-Ullrich JE, Bratton DL, Oldenborg PA, Michalak M, Henson PM (2005) Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte. Cell 123(2):321–334

    Article  CAS  Google Scholar 

  12. Gold LI, Eggleton P, Sweetwyne MT, Van Duyn LB, Greives MR, Naylor SM, Michalak M, Murphy-Ullrich JE (2010) Calreticulin: non-endoplasmic reticulum functions in physiology and disease. FASEB J 24(3):665–683

    Article  CAS  Google Scholar 

  13. Tarr JM, Young PJ, Morse R, Shaw DJ, Haigh R, Petrov PG, Johnson SJ, Winyard PG, Eggleton P (2010) A mechanism of release of calreticulin from cells during apoptosis. J Mol Biol 401(5):799–812

    Article  CAS  Google Scholar 

  14. Alur M, Nguyen MM, Eggener SE, Jiang F, Dadras SS, Stern J, Kimm S, Roehl K, Kozlowski J, Pins M, Michalak M, Dhir R, Wang Z (2009) Suppressive roles of calreticulin in prostate cancer growth and metastasis. Am J Pathol 175(2):882–890

    Article  CAS  Google Scholar 

  15. Machlus KR, Thon JN, Italiano JE Jr (2014) Interpreting the developmental dance of the megakaryocyte: a review of the cellular and molecular processes mediating platelet formation. Br J Haematol 165(2):227–236

    Article  Google Scholar 

  16. Elton CM, Smethurst PA, Eggleton P, Farndale RW (2002) Physical and functional interaction between cell-surface calreticulin and the collagen receptors integrin alpha2beta1 and glycoprotein VI in human platelets. Thromb Haemost 88(4):648–654

    Article  CAS  Google Scholar 

  17. Reilly D, Larkin D, Devocelle M, Fitzgerald DJ, Moran N (2004) Calreticulin-independent regulation of the platelet integrin alphaIIbbeta3 by the KVGFFKR alphaIIb-cytoplasmic motif. Platelets 15(1):43–54

    Article  CAS  Google Scholar 

  18. Dubois C, Panicot-Dubois L, Merrill-Skoloff G, Furie B, Furie BC (2006) Glycoprotein VI-dependent and -independent pathways of thrombus formation in vivo. Blood 107(10):3902–3906

    Article  CAS  Google Scholar 

  19. Jardín I, López JJ, Pariente JA, Salido GM, Rosado JA (2008) Intracellular calcium release from human platelets: different messengers for multiple stores. Trends Cardiovasc Med 18(2):57–61

    Article  Google Scholar 

  20. Wang Z, Li S, Shi Q, Yan R, Liu G, Dai K (2010) Calmodulin antagonists induce platelet apoptosis. Thromb Res 125(4):340–350

    Article  CAS  Google Scholar 

  21. Bishop-Bailey D (2010) The platelet as a model system for the acute actions of nuclear receptors. Steroids 75(8–9):570–575

    Article  CAS  Google Scholar 

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Funding

This work was supported by Natural Science Foundation of Guangdong Province, China (Nos. 9151802904000008 and S2011010004982).

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Authors and Affiliations

  1. Division of Hematology, The Second Affiliated Hospital of Shantou University Medical College, Dongxia Road North, Shantou, 515041, Guangdong Province, China

    Kam Chau Yung, Ze Wen Zhang, Wen Jun Yu, Chun Ling He, Xian Ru Xu & Jun Yin

  2. Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Dongxia Road North, Shantou, 515041, Guangdong Province, China

    Jun Yin

  3. Division of Respiration, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, China

    Jin Feng Qiu

  4. Blood Purification Room, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, China

    Cheng Wei Xu

  5. Translational Medicine Research Center, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, China

    Jun Yin

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  1. Kam Chau Yung
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  2. Ze Wen Zhang
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  3. Wen Jun Yu
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  4. Jin Feng Qiu
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  5. Cheng Wei Xu
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  6. Chun Ling He
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  7. Xian Ru Xu
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  8. Jun Yin
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Corresponding author

Correspondence to Jun Yin.

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This study was approved by the ethical review committee of the Second Affiliated Hospital of Shantou University Medical College.

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Yung, K.C., Zhang, Z.W., Yu, W.J. et al. Study on the Role of Calreticulin Within Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura. Indian J Hematol Blood Transfus 34, 711–718 (2018). https://doi.org/10.1007/s12288-018-0955-8

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  • DOI: https://doi.org/10.1007/s12288-018-0955-8

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